487923

Anti-Nitrotyrosine Mouse mAb (CC22.8C7.3)

liquid, clone CC22.8C7.3, Calbiochem^®

• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: CC22.8C7.3, monoclonal
• form: liquid
• contains: ≤0.1% sodium azide as preservative
• species reactivity (predicted by homology): all
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• isotype: IgG2b
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... NOS1(4842)

Description

General description

Purified mouse monoclonal antibody. Recognizes nitrotyrosine-containing proteins.

Recognizes nitrotyrosine.

This Anti-Nitrotyrosine Mouse mAb (CC22.8C7.3) is validated for use in Immunoblotting for the detection of Nitrotyrosine.

Immunogen

peroxynitrite-treated KLH

Application

Immunoblotting (2 µg/ml)

Warning

Toxicity: Standard Handling (A)

Physical form

In PBS, 50% glycerol, 0.1% BSA, 0.02% sodium azide, pH 7.2.

Reconstitution

Reconstitute with H₂O to a final stock concentration of 1 mg/ml. Following reconsitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.

Analysis Note

Positive Control
Mouse macrophages treated with peroxynitrite

Other Notes

Myatt, L., et al. 1996. Hypertension28, 488.
Beckman, J.S., et al. 1994. Biol. Chem. Hoppe-Seyler375, 81.

Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable