G005747
HPLC Analysis of Sugars on Ascentis® Si
application for HPLC
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About This Item
technique(s)
HPLC: suitable
test parameters
column: Ascentis Si, 15 cm x 4.6 mm I.D., 5 μm particles (581512-U)
mobile phase: [A] water; [B] acetonitrile; (15:85, A:B)
flow rate: 1.0 mL/min
column temp.: 35 °C
detector: ELSD, 55 °C, 3.5 bar nitrogen
injection: 10 μL
suitability
application for HPLC
application(s)
food and beverages
Analysis Note
This application demonstrates the suitability of the Ascentis Si for the analysis of sugars. Structures along with the optimized chromatogram obtained on the Ascentis Si are presented. Separation is by normal-phase mechanism as a decrease in mobile phase water content results in increased retention.
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Analyte
Description
Sucrose
Maltose
Maltotriose
analytical column
Product No.
Description
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