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Key Documents

G005713

LC/MS Analysis of Omeprazole and Metabolites on Ascentis® Express RP Amide (pH 2.7)

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: Ascentis Express RP-Amide, 5 cm x 2.1 mm I.D., 2.7 μm particles (53911-U)
mobile phase: [A] 10 mM ammonium formate in water, pH 2.7; [B] 10 mM ammonium formate in methanol, pH 2.7; (65:35, A:B)
flow rate: 0.3 mL/min
column temp.: 35 °C
detector: ESI(+), m/z 50-400
injection: 1 μL
sample: 1 mg/L in mobile phase

suitability

application for HPLC

application(s)

clinical

Analysis Note

This application demonstrates the suitability of the Ascentis Express RP Amide for the analysis of omeprazole metabolites.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    5-Hydroxyomeprazole
    Omeprazole
    Omeprazole sulfone

analytical column

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mobile phase component

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standard

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