G005662
HPLC Analysis of Aflatoxins on Ascentis® Express C18 with Fluorescence Detection
application for HPLC
About This Item
technique(s)
HPLC: suitable
test parameters
column: Ascentis Express C18, 15 cm x 4.6 mm I.D., 2.7 μm particles (53829-U)
mobile phase: [A] water; [B] acetonitrile; (75:25, A:B)
flow rate: 2.0 mL/min
pressure: 6381 psi (440 bar)
column temp.: 35 °C
detector: fluorescence, ex 360 nm, em 440 nm
injection: 50 μL
sample: 200 μL aflatoxin standard in acetonitrile; 0.5 μg/mL B2 and G2; 2 μg/mL B1 and G1; 800 μL delivatization solution: 70% water, 20% TFA and 10% acetic acid
heat for 9 min at 65 °C
suitability
application for HPLC
application(s)
food and beverages
Legal Information
Analyte
analytical column
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