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G005615

HPLC Analysis of Vitamin D2 and D3 on Ascentis® Express C18

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: Ascentis Express C18, 15 cm x 2.1 mm I.D., 2.7 μm particles (53825-U)
mobile phase: acetonitrile
flow rate: 0.15 mL/min
column temp.: 35 °C
detector: UV, 290 nm
injection: 10 μL
sample: 10 mg/L each (vitamins D2 and D3) in methanol

suitability

application for HPLC

application(s)

clinical
vitamins, nutraceuticals, and natural products

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description

    Ergocalciferol (D2), analytical standard
    Cholecalciferol (D3), analytical standard

standard

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