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G004524

HPLC Analysis of Carnitine Enantiomers on Astec® CHIROBIOTIC® TAG

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: CHIROBIOTIC TAG, 25 cm x 4.6 mm I.D., 5 μm particles (14024AST)
mobile phase: [A] 5 mM ammonium acetate, pH 6; [B] methanol; (25:75, A:B)
flow rate: 0.6 mL/min
column temp.: 35 °C
detector: ESI(+)-MS (m/z = 162)
injection: 5 μL
sample: 1 μg/mL in mobile phase

suitability

application for HPLC

application(s)

life science and biopharma

Legal Information

Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC

Analyte

Description
    (±)-Carnitine hydrochloride, ≥98% (TLC)

analytical column

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