ZRB2297

Anti-His tag Antibody, clone 1J13 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): His6-Tag, Histidine-Tag
• UNSPSC Code: 12352203

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: recombinant monoclonal
• description: clone 1J13 ; recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• purified by: using Protein A
• species reactivity: all
• packaging: antibody small pack of 25
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• technique(s): affinity binding assay: suitable; flow cytometry: suitable; immunocytochemistry: suitable; western blot: suitable
• isotype: IgG
• Protein ID accession no.: N/A
• UniProt accession no.: N/A
• greener alternative category: Aligned,
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry. This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1J13 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects His-tagged proteins.

Immunogen

KLH-conjugated linear peptide corresponding to the sequence GSGHHHHHHGSGGSG.

Application

Quality Control Testing

Evaluated by Western Blotting with His-ABP-tagged recombinant Human Adiponectin.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected His-ABP-tagged recombinant Human Adiponectin.

Tested Applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected His-ABP-tagged, recombinant Human SMCR8 protein.

Affinity Binding Assay: A representative lot of this antibody bound His-tagged, recombinant Human SLC12A4 protein with a KD of 7.8 x 10-6 in an affinity binding assay.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected His-tagged AKT1 in HeLa cells transfected to express His-tagged AKT1 protein.

Flow Cytometry (FC) Analysis: 1 µg of this antibody from a representative lot detected His-tagged AKT1 protein in one million HeLa cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

The His-tag is one of the most commonly used purification tags, with six or more consecutive histidine residues. It can be attached to either the N- or the C-terminus of recombinant proteins. Here histidine tends to strongly coordinate with metal ions, such as Ni2+ or Co2+ immobilized on beads or a resin for purification. Since this tag is small, it does not require removal before use of the recombinant protein, and it does not cause any significant interference with the function or structure of a majority of proteins. The His-tagged proteins can be easily detected or purified by used of anti-His-tag antibody. This antibody can also be used to monitor the progress of purification and detection of proteins using immunoblotting, immunostaining, and immunoprecipitation protocols. It can detect proteins with His-tag fused to either the N- or the C-terminus. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Booth, WT., et al. (2018). ACS Omega. 3(1); 760-768).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable