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ZMS1044

Sigma-Aldrich

Anti-53BP1 Antibody, clone BP13 ZooMAb® Mouse Monoclonal

enhanced validation
greener alternative

recombinant, expressed in HEK 293 cells

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About This Item

UNSPSC Code:
12352203

biological source

mouse

Quality Level

recombinant

expressed in HEK 293 cells

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

BP13, recombinant monoclonal
monoclonal

product line

ZooMAb® learn more

form

lyophilized

mol wt

calculated mol wt ~213.57 kDa
observed mol wt ~270 kDa

species reactivity

human

packaging

antibody small pack of 25 μL

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

isotype

IgG1

UniProt accession no.

greener alternative category

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Specificity

Clone BP13 is a ZooMAb® mouse recombinant monoclonal antibody that specifically detects human p53-binding protein 1.

Immunogen

Full-length recombinant human p53-binding protein 1.

Application

Quality Control Testing

Evaluated by Western Blotting in HeLa cell nuclear extract.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected TP53-binding protein 1 in HeLa cell nuclear extract.

Tested Applications

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected 53BP1 in human breast cancer tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected 53BP1 in HeLa cells treated with UV light.

Flow Cytometry Analysis: 1 μg from a representative lot detected 53BP1 in one million Hela cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-53BP1, clone BP13 ZooMAb®, Cat. No. ZMS1044, is a recombinant Mouse monoclonal antibody that targets TP53-binding protein 1 and is tested for use in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Target description

TP53-binding protein 1 (UniProt: Q12888; also known as 53BP1, p53-binding protein 1, p53BP1) is encoded by the TP53BP1 gene (Gene ID: 7158) in human. 53BP1 is a homooligomeric double-strand break (DSB) repair protein that is involved in response to DNA damage and promotes non-homologous end joining (NHEJ)-mediated repair. It counteracts the function of the homologous recombination (HR) repair protein BRCA1. In response to DSB it undergoes phosphorylation by ATM, which promotes interaction with RIF1 and dissociation from NUDT16L1/TIRR, leading to recruitment to DSBs sites where it binds histone H2A monoubiquinated at lysine 15 (H2AK15Ub) and histone H4 dimethylated at lysine 20 (H4K20me2). Its Tudor-like region (aa 1484-1603) mediates its binding to H4K20m3e2. In the absence of DNA damage, it is phosphorylated at basal level, but following DSB and phosphorylation by ATM it interacts with PAX-interacting protein 1 (PAXIP1) and non-homologous end joining of dysfunctional telomeres. Three isoforms of 53BP1 have been described that are produced by alternative splicing. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Panier, S., and Boulton, S. (2014). Nat Rev Mol Cell Biol 15(1); 7-18).

Physical form

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

11 - Combustible Solids

WGK

WGK 1


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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