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T6701

Sigma-Aldrich

TargeTron Vector pNL9164

100 ng/μL

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About This Item

UNSPSC Code:
41106609
NACRES:
NA.51

concentration

100 ng/μL

shipped in

wet ice

storage temp.

−20°C

General description

The TargeTron Vector pNL9164 is a 9,295 bp expression vector for use with the TargeTron Gene Knockout System (product number TA0100). This circularized vector can be used for targeted gene knockouts in gram-positive organisms such as Staphyloccus aureus. As supplied, the pNL9164 vector is re-targeted to the S. aureus hsa gene. The hsa gene encodes a member of the HU family of bacterial histone-like proteins. For validation in S. aureus, this vector can be used directly to knockout hsa without any further modifications.

Legal Information

TargeTron is a trademark of InGex, LLC

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Megan R Kiedrowski et al.
PloS one, 6(11), e26714-e26714 (2011-11-19)
Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) is an emerging contributor to biofilm-related infections. We recently reported that strains lacking sigma factor B (sigB) in the USA300 lineage of CA-MRSA are unable to develop a biofilm. Interestingly, when spent media from a

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