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Key Documents

SAB4503840

Sigma-Aldrich

Anti-phospho-WNK1 (pThr58) antibody produced in rabbit

affinity isolated antibody

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 250 kDa

species reactivity

rat, human, mouse

concentration

~1 mg/mL

technique(s)

ELISA: 1:5000
immunohistochemistry: 1:50-1:100
western blot: 1:500-1:1000

NCBI accession no.

Q9H4A3

UniProt accession no.

Q9H4A3

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

phosphorylation (pThr58)

Gene Information

human ... WNK1(65125)

General description

The WNK1 (WNK lysine deficient protein kinase 1) gene is mapped to human chromosome 12p13.33. The encoded protein belongs to the WNK kinase family and is ubiquitously expressed.

Immunogen

The antiserum was produced against synthesized peptide derived from human WNK1 around the phosphorylation site of Thr58.

Immunogen Range: 24-73

Biochem/physiol Actions

WNK1 (WNK lysine deficient protein kinase 1) is responsible for maintaining the ion homeostasis. It regulates mitogen-activated protein kinase 7 (MAPK7) mediated cell proliferation and migration. It is also known to promote tumor proliferation, invasion and metastasis in prostate carcinoma. WNK1 expression is essential for vascular remodeling and endothelial sprouting. Autophagy has been indicated in various diseases. This intracellular degradation pathway is prevented by WNK1.(3)

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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A novel homozygous mutation in the WNK1/HSN2 gene causing hereditary sensory neuropathy type 2.}
Potulska-Chromik A, et al.
Acta Biochimica Polonica, 59(3) (2012)
WNK lysine deficient protein kinase 1 regulates human endometrial stromal cell decidualization, proliferation, and migration in part through mitogen-activated protein kinase 7
Adams NR, et al.
Biology of Reproduction, 97(3), 400-412 (2017)
Multistep regulation of autophagy by WNK1
Gallolu Kankanamalage S, et al.
Proceedings of the National Academy of Sciences of the USA, 113(50), 14342-14347 (2016)
Stefanie Albrecht et al.
Glia, 67(5), 870-883 (2019-01-10)
In multiple sclerosis, demyelination occurs as a consequence of chronic autoimmunity in the central nervous system causing progressive neurological impairment in patients. After a demyelinating event, new myelin sheaths are formed by adult oligodendroglial progenitor cells; a process called remyelination.

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