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P2949

Sigma-Aldrich

PIPES sodium salt

≥99% (titration)

Synonym(s):

Na-PIPES, Piperazine-1,4-diethanesulfonate sodium salt, 1,4-Piperazinediethanesulfonic acid sodium salt, Piperazine-N,N′-bis(2-ethanesulfonic acid) sodium salt

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About This Item

Linear Formula:
C8H17N2O6S2Na
CAS Number:
10010-67-0
Molecular Weight:
324.35
EC Number:
233-005-3
MDL number:
MFCD00065472
UNSPSC Code:
12161700
PubChem Substance ID:
24898375
NACRES:
NA.25

Assay

≥99% (titration)

form

crystalline powder

pH

4.5-4.7

useful pH range

6.1-7.5

pKa (25 °C)

6.8

solubility

0.1 M NaOH: 250 mg/mL, clear, colorless

application(s)

diagnostic assay manufacturing

storage temp.

room temp

SMILES string

[Na+].OS(=O)(=O)CCN1CCN(CC1)CCS([O-])(=O)=O

InChI

1S/C8H18N2O6S2.Na/c11-17(12,13)7-5-9-1-2-10(4-3-9)6-8-18(14,15)16;/h1-8H2,(H,11,12,13)(H,14,15,16);/q;+1/p-1

InChI key

OGGAIRCLBMGXCZ-UHFFFAOYSA-M

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General description

1, 4-piperazinediethanesulfonic acid (PIPES) is a zwitterionic buffer that belongs to the group of the ethanesulfonic acid buffer series, first introduced by Good et al., It is a non-toxic buffer used in cell culture and tissue fixation for electron microscopy and other biological applications. PIPES don’t interact with metal ions. Since its pKa at 37°C is near physiological pH, it has been used as a buffering substance in biological, biochemical, and environmental studies. PIPES is developed to meet certain criteria: midrange pKa, maximum water solubility and minimum solubility in all other solvents, minimal salt effects, minimal change in pKa with temperature, chemically and enzymatically stable, minimal absorption in visible or UV spectral range and easily synthesized. Buffers can be prepared by adding a solution of the base to PIPES-free acid, titrating to the appropriate pH, or by mixing equimolar solutions of the monosodium salt and the disodium salt, titrating to the appropriate pH.

Application

PIPES sodium salt buffer has been used:

  • to prepare BRB80 buffer for imaging dynamic microtubules and single kinesin molecules through fluorescence microscopy
  • as a component of PEM fixation solution
  • to reconstitute red blood cells (RBCs) to study the effect of pH on 5-coordinate alpha-nitrosyl-hemoglobin (HbNO) stability

Features and Benefits

  • Non-toxic
  • Easy to use
  • Thermally stable
  • Easy to handle and store

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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B W Nielsen et al.
Agents and actions, 35(3-4), 170-178 (1992-03-01)
Increased osmotic pressure has been reported to cause non-cytotoxic histamine release (HR) from human basophils, as well as a potentiation of HR induced by anti-IgE. In this study, the effects of hyperosmolar Na-K-acetate (300-600 mOsm/kg H2O) on HR was studied
E R Swenson et al.
The American journal of physiology, 250(2 Pt 2), F288-F293 (1986-02-01)
Maximal rates of renal hydrogen ion secretion and bicarbonate reabsorption in the dogfish were stimulated by intravascular infusion of acidic and basic buffers: bicarbonate, phosphate, phenol red, dimethadione (DMO), imidazole, and piperazine-N,N'-bis(2 ethanesulfonic acid) (PIPES). There was no difference in
Rajesh K Sani et al.
Environmental science & technology, 39(7), 2059-2066 (2005-05-06)
In cultures of Desulfovibrio desulfuricans 620 the effects of iron(III) (hydr)oxides (hematite, goethite, and ferrihydrite) on microbial reduction and reoxidation of uranium (U) were evaluated under lactate-limited sulfate-reducing conditions. With lactate present, G20 reduced U(VI) in both 1,4-piperazinediethanesulfonate (PIPES) and
R L Schultz et al.
Journal of neurocytology, 15(4), 461-469 (1986-08-01)
When used for vascular perfusion of brain, 0.1 M PIPES-buffered 3% glutaraldehyde resulted in the formation of expanded, vesicle-filled cell processes limited by multiple membrane layers. These structures, termed multivesicular myelin figures and interpreted as artefacts, were most common in
V D Prokopieva et al.
Alcohol and alcoholism (Oxford, Oxfordshire), 35(1), 44-48 (2000-02-24)
The effects of carnosine and related compounds on erythrocytes from alcoholics were studied. In their presence, erythrocytes showed an increased ability to resist haemolysis and showed a more normal morphology, with carnosine and N-acetyl-carnosine being the most effective compounds. These
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