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N3643

Sigma-Aldrich

NZCYM Broth

Powder microbial growth medium

Synonym(s):

Broth medium

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About This Item

UNSPSC Code:
41106200
NACRES:
NA.85

grade

for molecular biology

sterility

non-sterile

form

powder

composition

Casamino Acids, 1 g/L
MgSO4, 0.98 g/L
NaCL, 5 g/L
Tryptone, 10 g/L
Yeast extract, 5 g/L

technique(s)

microbiological culture: suitable

pH

6.8-7.2(2.2% solution)

application(s)

food and beverages
microbiology

storage temp.

room temp

suitability

nonselective for Escherichia coli
nonselective for coliforms

Related Categories

General description

NZCYM is a microbial growth medium used for the cultivation of E. coli and lambda bacteriophage. This nutrient-rich microbial broth contains peptides, amino acids, and water-soluble vitamins in a low-salt formulation.

Application

NZCYM Broth has been used for non-selective cultivation of lambda, MS2, T7, CLP-P3, Phi29, and Goe1 bacteriophages, and E. coli strains for cloning, DNA plasmid production, and production of recombinant proteins. It is also suitable for selective cultivation when appropriate antibiotics are added.

Features and Benefits

NZCYM powder provides:
  • Standard formulation
  • Convenient package sizes
  • A budget-friendly alternative to liquid

Reconstitution

Stir to suspend 22 g powder in 1L water. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Shane R Wilkinson et al.
The Journal of biological chemistry, 277(19), 17062-17071 (2002-02-14)
Trypanosoma cruzi glutathione-dependent peroxidase I (TcGPXI) can reduce fatty acid, phospholipid, and short chain organic hydroperoxides utilizing a novel redox cycle in which enzyme activity is linked to the reduction of trypanothione, a parasite-specific thiol, by glutathione. Here we show
Bioreagents for Cloning
Sigma data, 2-2 (2011)
Chris Coward et al.
Applied and environmental microbiology, 72(7), 4638-4647 (2006-07-06)
This study characterizes the interaction between Campylobacter jejuni and the 16 phages used in the United Kingdom typing scheme by screening spontaneous mutants of the phage-type strains and transposon mutants of the sequenced strain NCTC 11168. We show that the
Vinni Mona Hansen et al.
BMC microbiology, 7, 90-90 (2007-10-20)
The predominant food borne pathogen in the western world today is Campylobacter. Campylobacter specific bacteriophages (phages) have been proposed as an alternative agent for reducing the burden of Campylobacter in broilers. One concern in relation to phage biocontrol is the
Carla M Carvalho et al.
BMC microbiology, 10, 232-232 (2010-09-03)
Poultry meat is one of the most important sources of human campylobacteriosis, an acute bacterial enteritis which is a major problem worldwide. Campylobacter coli and Campylobacter jejuni are the most common Campylobacter species associated with this disease. These pathogens live

Protocols

Microbial Growth Protocols

General protocols for growth of competent cells in microbial medium.

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