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M5310

Sigma-Aldrich

Mineral oil

light oil, suitable for mouse embryo cell culture, BioXtra

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About This Item

CAS Number:
8042-47-5
EC Number:
232-455-8
MDL number:
MFCD00131611
UNSPSC Code:
12352207
NACRES:
NA.75

sterility

sterile-filtered

product line

BioXtra

form

light oil

storage condition

protect from light

technique(s)

cell culture | embryo: suitable

refractive index

n20/D 1.467 (lit.)

density

0.82-0.88 g/mL at 25 °C

format

neat

InChI key

AEOVEGJBKQQFOP-DDVLFWKVSA-L

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General description

Mineral oil or liquid petroleum is used for conservation of various types of cutltures. The advantages of using mineral oil are that the growth rate is decreased and the evaporation of water from the medium is almost completely eliminated. Thus, sub-culturing is reduced leading to increase in the viability of the culture. The first time mineral oil was used was in 1914, and it was used to overlay bacterial culture.

Application

Mineral oil has been commonly used and recommended for overlaying mouse embryo cultures to prevent drying during the procedure.
Mineral oil is used for the following applications:
  • Used for cell viability studies in preparing the culture media or media supplements
  • Used tp protect array from evaporation by adding mineral oil around the array
  • Used to prepare the needle for microinjection (microinjection needle pulling, sharpening, and loading)
  • Used in PCR setup

Features and Benefits

Ready-to-use sterile filtered product conveniently packaged in PET bottles.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

No data available

Flash Point(C)

No data available

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Slobodan Beronja et al.
Methods in molecular biology (Clifton, N.J.), 961, 351-361 (2013-01-18)
We have recently developed a method for RNAi-mediated gene function analysis in skin (Beronja et al., Nat Med 16:821-827, 2010). It employs ultrasound-guided in utero microinjections of lentivirus into the amniotic cavity of embryonic day 9 mice, which result in
Mineral Oil Overlay for Conservation of Plant Tissue Cultures
Caplin SM
American Journal of Botany, 46(5), 324-329 (1959)
Hanyoup Kim et al.
Lab on a chip, 9(9), 1230-1235 (2009-04-17)
We report high-speed real-time PCR performed on an unmodified disposable polystyrene Petri dish. The reaction cycle relies solely on an infrared laser for heating; no conventional heater is required. Nanoliter droplets of PCR mixture as water-in-oil emulsions printed in an
Bei Zhao et al.
Scientific reports, 7(1), 11319-11319 (2017-09-14)
A microfluidic chip with a controllable and integrated piezoelectric pump was proposed and demonstrated, where the pump was designed as a micro-actuator based on polyvinylidene fluoride (PVDF) organic piezoelectric film. In this case, the pump should integrate with the microfluidics
Hanyoup Kim et al.
Optics express, 17(1), 218-227 (2009-01-09)
We report the successful application of low-power (approximately 30 mW) laser radiation as an optical heating source for high-speed real-time polymerase chain reaction (PCR) amplification of DNA in nanoliter droplets dispersed in an oil phase. Light provides the heating, temperature
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Protocols

Determination of Water Content in Mineral oil Using Karl Fischer Titration

Summary application report for analysis of moisture in Mineral oil

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