K4894

Poloxamer 188

solid

• Synonym(s): Poly(ethylene glycol)-block-poly(propylene glycol)-block-poly(ethylene glycol), Lutrol F68
• CAS Number: 9003-11-6
• MDL number: MFCD00082049
• UNSPSC Code: 12161902
• NACRES: NA.75

Properties

• Agency: USP/NF; meets USP testing specifications
• form: solid
• mol wt: 8.400 g/mol
• pH: 5.0-7.5(2.5% solution)
• application(s): sample preservation
• InChI: 1S/C3H6O.C2H4O/c1-3-2-4-3;1-2-3-1/h3H,2H2,1H3;1-2H2
• InChI key: RVGRUAULSDPKGF-UHFFFAOYSA-N

Description

General description

Poloxamer 188 is a non-ionic amphiphilic copolymer, which has a central chain of hydrophobic polyoxypropylene and a hydrophilic polyoxyethylene at the ends.

Application

Poloxamer 188 has been used as a component of cell-dissociation/minimal essential media (MEM) to sort tumor cells using EpCAM antibody-conjugated magnetic beads.

Poloxamer 188 has been used as a supplement in F17 medium for the culture of HEK293-6E cells. It has also been used as a component of flow cytometry (FACS) buffer as a non-ionic detergent that protects cells from hydrodynamic damage.

Biochem/physiol Actions

Poloxamer 188 exhibits cytoprotective, hemorheological, anti-inflammatory, antithrombotic activities. It maintains the stability of membrane barrier function. P188 lowers surface tension and hydrophobic-based cellular adherence. It also inhibits lipid peroxidation stimulated by Fe²⁺ and H₂O₂.

Legal Information

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1