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G5507

Sigma-Aldrich

Galactosyltransferase from bovine milk

lyophilized powder

Synonym(s):

Lactose Synthase, UDP-galactose:D-glucose 4-β-galactosyltransferase

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About This Item

CAS Number:
9031-68-9
Enzyme Commission number:
2.4.1.22 (BRENDA, IUBMB)
MDL number:
MFCD00131122
UNSPSC Code:
12352204
NACRES:
NA.54

form

lyophilized powder

Quality Level

200

specific activity

3.0-15.0 units/mg protein (in the presence of adequate α-lactalbumin, without added α-lactalbumin the activity is zero)

composition

Protein, 35-65% modified Warburg-Christian

impurities

≤10 % protein α-lactalbumin

storage temp.

−20°C

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General description

Research area: Cell signalling. Galactosyltransferase(GalT) is found in both prokaryotes and eukaryotes.Galactosyltransferases belong to the galactosyltransferase family and are anchored to the membrane of Golgi through their N-terminal region.

Application

Galactosyltransferase from bovine milk has been used:
  • in the generation of hyper-galactosylated variant of IgG1
  • in β -1,4-galactosyltransferase assay rice root cell extracts
  • in the modification of LacNAcQ11 and LacDiNAcQ11 nanofibers

Galactosyltransferase is the catalytic component of the lactose synthetase system; it synthesizes lactose slowly in the absence of the regulatory protein α-lactalbumin. Galactosyltransferase will also transfer galactose from UDP-galactose to N-acetylglucosamine. This preparation is useful in the determination of α-lactalbumin, UDP-galactose, and N-acetylglucosamine.

Biochem/physiol Actions

Galactosyltransferase catalyzes the transfer of galactosyl molecules in the synthesis of oligosaccharides.
Galactosyltransferase from bovine milk requires Mn2+ ions for its catalytic functionality. Alteration in the galactosyltransferase levels have been implicated in various disease states.Galactosyltransferase(GalT) functions either as a soluble enzyme or as membrane-bound moieties.

Linkage

This is probably the same enzyme as N-acetyllactosamine synthetase, EC 2.4.1.38 and EC 2.4.1.90.

Unit Definition

One unit will transfer 1.0 μmole of galactose from UDP-galactose to D-glucose per min at pH 8.4 at 30 °C in the presence of 0.2 mg α-lactalbumin per mL of reaction mixture. (without added α-lactalbumin the activity is zero)

Physical form

Lyophilized powder containing Tris, EDTA, and (NH4)2SO4

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Structure and function of beta-1, 4-galactosyltransferase
Qasba PK, et al.
Current Drug Targets, 9(4), 292-309 (2008)
Hierarchical self-assembly and emergent function of densely glycosylated peptide nanofibers
Restuccia A, et al.
Communications Chemistry, 2(1), 53-53 (2019)
In vitro glycoengineering of IgG1 and its effect on Fc receptor binding and ADCC activity
Thomann M, et al.
PLoS ONE, 10(8), e0134949-e0134949 (2015)
A classification of nucleotide-diphospho-sugar glycosyltransferases based on amino acid sequence similarities.
J A Campbell et al.
The Biochemical journal, 326 ( Pt 3), 929-939 (1997-10-23)
A novel short-root gene encodes a glucosamine-6-phosphate acetyltransferase required for maintaining normal root cell shape in rice
Jiang H, et al.
Plant Physiology, 138(1), 232-242 (2005)
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Articles

Glycosyltransferases

Glycosyltransferases were initially considered to be specific for a single glycosyl donor and acceptor, which led to the one enzyme-one linkage concept. Subsequent observations have refuted the theory of absolute enzymatic specificity by describing the transfer of analogs of some nucleoside mono- or diphosphate sugar donors.

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