G1401
Glial Cell Line-derived Neurotrophic Factor from rat
recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture, ≥97% (SDS-PAGE)
Synonym(s):
ATF, Astrocyte-derived trophic factor, GDNF
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About This Item
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biological source
rat
Quality Level
recombinant
expressed in baculovirus infected Sf21 cells
Assay
≥97% (SDS-PAGE)
form
lyophilized powder
potency
0.10-10 ng/mL
mol wt
~30 kDa
packaging
pkg of 10 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
UniProt accession no.
storage temp.
−20°C
Gene Information
rat ... Gdnf(25453)
Biochem/physiol Actions
Glial cell-derived neurotrophic factor (GDNF) is a neurotrophic factor that is a member of the TGF-β superfamily. GDNF is founding member of the GDNF family of ligands, which to date include GDNF, neurturin (NTN), persephin (PSP) and artemin (ART). GDNF is a glycosylated disulfide-linked homodimeric protein of ~15 kDa. Mature rat and human GDNF share 93% sequence homology with strong species cross-reactivity. GDNF promotes survival of various neuronal cells in central and peripheral nervous systems and different stages of development including motoneurons, midbrain dopaminergic neurons, Purkinje cells, and sympathetic neurons. Cells known to express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes, and skeletal muscle cells. In addition, exogenously applied GDNF has been shown to rescue damaged facial motor neurons in vivo.
Physical form
Lyophilized from 50 μL of a 0.2 um filtered solution in PBS, pH 7.4 with 50 ug BSA per 1 ug as a carrier protein
Analysis Note
The biological activity of GDNF is measured by its ability to bind to immobilized rrGFRa1/Fc in a functional ELISA.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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