DUO92020
Duolink® In Situ PLA® Probe Anti-Human PLUS
Affinity purified Donkey anti-Human IgG (H+L)
Synonym(s):
in situ Proximity Ligation Assay, Protein Protein Interaction Kit
About This Item
biological source
donkey (Polyclonal)
antibody form
affinity purified immunoglobulin (Secondary antibody)
product line
Duolink®
species reactivity
human
technique(s)
immunofluorescence: suitable
proximity ligation assay: suitable
suitability
suitable for brightfield
suitable for fluorescence
shipped in
wet ice
storage temp.
2-8°C
Application
This product can be applied to both the Duolink® In Situ Fluorescence Protocol and the Duolink® In Situ Brightfield Protocol depending on the detection reagents used.
Visit our Duolink® PLA Resource Center for information on how to run a Duolink® experiment, applications, troubleshooting and more.
To perform a complete Duolink® PLA® in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes. Other necessary reagents include a pair of PLA probes from different species (one PLUS and one MINUS), detection reagents, wash buffers, and mounting medium. Note that the primary antibodies must come from the same species as the Duolink® PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.HRP is also available for brightfield detection.
PLA probe anti-Human reacts with whole molecule human IgG and the light chains of other human immunoglobulin?s. The PLA Probe Anti-Human Plus has minimal cross-reactivity with bovine, chicken, goat, guinea pig, Syrian hamster, horse, mouse, rabbit, rat, and sheep serum proteins. A MINUS probe of a different species must be used simultaneously with this product. See our Product Selection Guide for more information.
Application Note
Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink® PLA in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.
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Features and Benefits
- No overexpression or genetic manipulation required
- High specificity (fewer false positives)
- Single molecule sensitivity due to rolling circle amplification
- Relative quantification possible
- No special equipment needed
- Quicker and simpler than FRET
- Increased accuracy compared to co-IP
- Publication-ready results
Components
- 5x PLA Probe Anti-Human PLUS - Donkey anti-human secondary antibody conjugated to oligonucleotide PLUS
- 1x Blocking Solution - Reagent for blocking of the sample
- 1x Antibody Diluent - For dilution of PLA probes and primary antibodies
See datasheet for more information.
Legal Information
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 2 - Skin Sens. 1
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Protocols
Protocol for use of Duolink® PLA reagents for the detection of individual proteins, protein modifications, and protein-protein interactions within cell populations by flow cytometry.
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