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product name
TM4, 88111401
biological source
mouse testis
growth mode
Adherent
karyotype
Not specified
morphology
Not specified
products
Transferrin, H-Y Ag, novel retinol-binding protein
receptors
Androgen, oestrogen and progesterone receptors
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse BALB/c testis sertoli cell
Cell Line Description
From primary culture of sertoli cell enriched preparations from normal testes of a 11-13 day old BALB/c nu/+ mice. Cells respond to FSH but not LH; Plasminogen activator secretion is stimulated by FSH and also retinoic acid. Note: only distributed frozen.
Application
Hormone studies
Culture Medium
Ham′s F12 + DMEM (1:1) + 2mM Glutamine + 5% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:20 to 1:200 i.e. seeding at 1x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Cells resuscitated from frozen stock should be split 1:3 at the first passage and thereafter as above.
Other Notes
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Certificates of Analysis (COA)
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