Recommended Products
product name
CNC 127I, 87091701
biological source
mouse breast
growth mode
Adherent
karyotype
Not specified
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse RIII mammary tumour
Cell Line Description
Subclone of parent line C127. The cells support the growth of Bovine Papilloma Virus and have been used for DNA transfection studies.
Application
Transfection and virus studies: Bovine papilloma virus
Culture Medium
DMEM + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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Certificates of Analysis (COA)
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