OAW42

85073102

• Synonym(s): OAW-42 Cells
• UNSPSC Code: 41106514

Properties

• biological source: human ovary
• growth mode: Adherent
• karyotype: 2n = 86
• morphology: Epithelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human ovarian tumour epithelial

Cell Line Description

From an ascites of patient with ovarian cystadenocarcinoma. Has retained ability to form free floating cysts in vitro and produces extracellular matrix. Has defined chemosensitivity pattern. Valuable for studies on biology of human ovarian cancer.

Application

OAW42 has been used to investigate the cytotoxic potential of histone deacetylase 1 as a target for ovarian cancer immunotherapy and to study the effects of tazemetostat, a potent and selective EZH2 inhibitor, on OAW42 cell lines.

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 11
D16S539: 12,13
D5S818: 11,12
D7S820: 8
THO1: 6,7
TPOX: 8,11
vWA: 15,16

Culture Medium

DMEM + 2mM Glutamine + 1mM Sodium Pyruvate (NaP) + 20 IU/l Bovine Insulin + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C.

Other Notes

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