Recommended Products
biological source
human skin
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Human malignant melanoma
Cell Line Description
Malignant Melanoma. Cell Bank Australia reference number: CBA1349.
DNA Profile
Not specified
Culture Medium
RPMI 1640 (with 2 mM L-Glutamine + 25 mM HEPES) +10% FBS.
Subculture Routine
Optimal split ratio 1:4 (seeding density 1.2 x104 cells/cm2).Cells incubated at 37 °C with 5% CO2.Harvest the cells using 0.05% Trypsin/EDTA at 37 °C for 5 min.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
Depositor and originator: Peter Parsons - Queensland Institute of Medical Research, Australia
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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