BCA1
Bicinchoninic Acid Kit for Protein Determination
for 200-1000 μg/ml protein
Synonym(s):
BCA protein assay
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About This Item
UNSPSC Code:
12352106
NACRES:
NA.32
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Application
Bicinchoninic Acid Kit has been used for the protein estimation in salivary proteome samples, human embryonic kidney 293 cells and luteal cells.
Features and Benefits
- Simple, sensitive colorimetric assay for proteins
- Two stable reagents are used for the working solution
- Faster and easier than the Lowry protein assay
- Compatible with many detergents both ionic and nonionic
- Less variation between different proteins than the Bradford dye-binding assay
- Adaptable for use in microwell plates
Principle
Proteins reduce alkaline Cu(II) to Cu(I) in a concentration-dependent manner. Bicinchoninic acid is a highly specific chromogenic reagent for Cu(I), forming a purple complex with an absorbance maximum at 562 nm. The absorbance is directly proportional to protein concentration. This is an alternative to the Folin-Ciocalteu reagent for protein determination.
Kit Components Only
Product No.
Description
- Bicinchoninic Acid Solution 1 L
- 4%(w/v) CuSO4 · 5H2O Solution 25 mL
- Protein Standard Solution 5 x 1
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Met. Corr. 1
Storage Class Code
8A - Combustible corrosive hazardous materials
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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