B7151
Anti-Mouse IgG (Fab specific)–Biotin antibody produced in goat
affinity isolated antibody, buffered aqueous solution
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About This Item
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biological source
goat
Quality Level
conjugate
biotin conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
technique(s)
direct ELISA: 1:200,000-1:300,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200
western blot: 1:400,000-1:800,000 using total cell extract of HeLa cells
storage temp.
−20°C
target post-translational modification
unmodified
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General description
IgG (immunoglobulin G) antibody subtype is a predominant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids. IgG is found in four subclasses IgG1, IgG2, IgG3 and IgG4. IgG has a monomeric structure.
The product binds to all mouse Igs
Application
Anti-Mouse IgG (Fab specific)-Biotin antibody produced in goat was used in immunohistochemistry of lateral and medial menisci of rabbits and human adipose-derived stem cells.
Biochem/physiol Actions
IgG (immunoglobulin G) antibody participates in complement fixation and opsonization.
IgG antibody provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of biotin to Anti-Mouse IgG (Fab specific) antibody allows for the binding of various labels such as avidin or streptavidin.
Other Notes
Antibody adsorbed with bovine, equine and human serum proteins.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Preparation Note
Adsorbed to reduce background staining with bovine, horse or human samples.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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