A4464
Enhanced Avian Reverse Transcriptase [eAMV™ RT]
For reverse transcription at higher temperatures & rare mRNAs
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About This Item
Quality Level
form
crystalline powder
usage
sufficient for 50 reactions
feature
dNTPs included: no
hotstart: no
concentration
20 units/μL
technique(s)
RT-PCR: suitable
color
colorless
input
purified RNA
shipped in
wet ice
storage temp.
−20°C
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General description
eAMV™ Reverse Transcriptase is an enhanced form of Avian Myeloblastosis Virus (AMV) RT that synthesizes a DNA strand complementary to RNA, DNA, or an RNA:DNA hybrid. This exceptionally robust AMV RT has greater thermostability than standard AMV or Moloney murine leukemia virus(M-MLV) reverse transcriptase. eAMV™ RT is an ideal enzyme for producing high-quality full-length cDNA from total RNA or poly(A)+ RNA and is also efficient at transcribing long targets.
Application
Enhanced Avian Reverse Transcriptase (eAMV RT) is used to transcribe RNA into DNA, and facilitates efficient mRNA template driven sysnthesis of cDNAs. This is due to the abillity of this enhanced AMV-RT to transcribe large mRNA templates, to transcribe through difficult secondary structures, and to detect low abundance mRNAs by RT-PCR.
Enhanced Avian Reverse Transcriptase [eAMV™ RT] has been used for reverse transcription of total RNA to synthesize cDNA during quantitative reverse transcription PCR (RT-qPCR) analysis.
Biochem/physiol Actions
Reverse transcriptase catalyzes RNA template incorporation of dNTPs into complimantary DNA through phosphodiester bond formation.
Features and Benefits
- Greater sensitivity for low abundance mRNA
- Unsurpassed transcription through difficult secondary structures at elevated temperatures (up to 65°C)
- Efficient generation of full-length cDNA, up to 14.1 kb
- Produces first strand cDNA ready for PCR amplification
Packaging
Provided with a vial of 10× reaction buffer.
Unit Definition
One unit incorporates one nanomole of TMP into TCA precipitable material in 10 min using polyadenylic acid as template and oligo(dT)12-18 as a primer.
Legal Information
Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler.
eAMV is a trademark of Sigma-Aldrich Co. LLC
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 5-5 (1989)
C Tosh et al.
Acta virologica, 41(3), 153-155 (1997-06-01)
A method of reverse transcription (RT) and polymerase chain reaction (PCR) amplification of 1D (VP1) gene of foot-and-mouth disease (FMD) virus using one reaction mixture containing both avian myeloblastosis virus (AMV) reverse transcriptase (RTase) and Tfl DNA polymerase is described.
Emily Schifano et al.
Virulence, 10(1), 1013-1025 (2019-11-28)
Calcium signaling can elicit different pathways involved in an extreme variety of biological processes. Calcium levels must be tightly regulated in a spatial and temporal manner in order to be efficiently and properly utilized in the host physiology. The Ca2+-ATPase
K Dukas et al.
Analytical biochemistry, 215(1), 66-72 (1993-11-15)
We developed a reverse transcription-polymerase chain reaction (RT-PCR) method which permits the simultaneous amplification of several mRNAs, even though their relative levels may be very different. First-strand cDNAs were synthesized from total RNA by MMLV reverse transcriptase with oligo(dT)15 priming.
Y M Zhu et al.
British journal of cancer, 91(11), 1970-1976 (2004-11-17)
Interleukin-8/CXCL8 (IL-8) is a chemokine and angiogenic factor. Recently, IL-8 was identified as an autocrine growth factor in several human cancers. Here, we investigated the expression and function of IL-8 in lung cancer cells. The expressions of IL-8 and its
Articles
One approach to the analysis of gene expression is to measure the concentration of mRNA of a gene. There are several challenges to such analyses, such as the differences in half life between different transcripts, the temporal patterns of transcription and the lack of correlation between mRNA and protein.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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