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A4161

Sigma-Aldrich

Bovine Serum Albumin

lyophilized powder, essentially globulin free, BioReagent, suitable for cell culture

Synonym(s):

Albumin bovine serum, BSA, Bovine albumin

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About This Item

CAS Number:
9048-46-8
EC Number:
232-936-2
MDL number:
MFCD00130384
UNSPSC Code:
12352202
NACRES:
NA.27

biological source

bovine

Quality Level

200

product line

BioReagent

Assay

≥99%

form

lyophilized powder

mol wt

~66 kDa

purified by

cold ethanol and heatshock fractionation

packaging

poly bottle of

origin

USA origin

technique(s)

cell culture | mammalian: suitable

impurities

essentially globulin free

pH

7

solubility

water: soluble (50 mg/ml)

UniProt accession no.

P02769

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

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Application

Select the right albumin for your application with the Albumin Selection Guide.

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Features and Benefits

  • Tested for use in cell culture
  • Essentially globulin free

Preparation Note

Purified by a combination of heat shock and ethanol fractionation
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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Articles

Albumin in Cell Culture

This page segues to comprehensive insights on how serum albumin and other important cell culture components affect the performance of serum-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies. The page introduces the in vitro chemistry and biochemistry of albumin. These insights may also be applied to ex vivo and tissue engineering applications.

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