57000

Indigo carmine

for microscopy (Bact., Hist.), indicator (pH 11.5-14.0)

• Synonym(s): Acid Blue 74, Indigo-5,5′-disulfonic acid disodium salt, Indigocarmine
• Empirical Formula (Hill Notation): C₁₆H₈N₂Na₂O₈S₂
• CAS Number: 860-22-0
• Molecular Weight: 466.35
• Colour Index Number: 73015
• Beilstein: 4103904
• EC Number: 212-728-8
• MDL number: MFCD00005723
• UNSPSC Code: 12171500
• PubChem Substance ID: 57651616
• NACRES: NA.47

Properties

• grade: for microscopy (Bact., Hist.); indicator (pH 11.5-14.0)
• Quality Level: 100
• form: powder
• technique(s): microbe id | staining: suitable
• loss: ≤5% loss on drying
• pH: 3.87 (30 °C, 7.14 g/L)
• mp: >340 °C
• solubility: methanol: 0.01 g/10 mL, blue to very deep blue
• density: 0.71 g/cm³ at 29 °C
• εmax: ≥350 at 603-613 nm
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [Na+].[Na+].[O-]S(=O)(=O)c1ccc2NC(\C(=O)c2c1)=C3\Nc4ccc(cc4C3=O)S([O-])(=O)=O
• InChI: 1S/C16H10N2O8S2.2Na/c19-15-9-5-7(27(21,22)23)1-3-11(9)17-13(15)14-16(20)10-6-8(28(24,25)26)2-4-12(10)18-14;;/h1-6,17-18H,(H,21,22,23)(H,24,25,26);;/q;2*+1/p-2/b14-13+;;
• InChI key: KHLVKKOJDHCJMG-QDBORUFSSA-L

Description

Application

Indigo carmine has been used in agarose plates to study behavioral feeding assays in Drosophila larvae. It has also been used for macroscopic assessment of intestinal adenomatous polyps isolated from mice.

Biochem/physiol Actions

Indigo carmine is a contrast dye which helps in highlighting mucosal changes. It accumulates in crevices, cysts and foveolae and stains the tissue blue. The abnormal tissue remains translucent pink.

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H317
• Precautionary Statements: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves