36098

Anti-Rabbit IgG F(ab′)2 fragment - Atto 488, whole molecule antibody produced in goat

1 mg/mL protein

• Synonym(s): Atto 488-[F(ab′)2 fragment goat-Anti-rabbit IgG], whole molecule
• UNSPSC Code: 12352203
• NACRES: NA.46

Properties

• conjugate: Atto 488 conjugate
• Quality Level: 100
• antibody form: F(ab′)₂ fragment of affinity isolated antibody
• antibody product type: secondary antibodies
• clone: polyclonal
• contains: 50% glycerol as stabilizer
• species reactivity: rabbit
• concentration: 1 mg/mL protein
• fluorescence: λ_ex 500 nm; λ_em 522 nm in PBS
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

IgGs are glycoprotein antibodies that modulate several immune responses. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins. Goat anti-rabbit IgGs associate with rabbit IgGs.

Immunogen

rabbit IgG F(ab′)2 fragment

Application

Atto 488-goat anti-rabbit IgG F(ab′)2 fragment, whole molecule antibody can be used for fluorescence-based immunoassays.

Physical form

Atto 488 F(ab)′2 fragment goat anti-rabbit IgG (whole molecule) is provided in unit sizes of 1 ml as 1mg/mL solutions in 0.1 M sodium phosphate, 0.1 M NaCl, pH 7.5, containing 5 mM sodium azide as a preservative.

Preparation Note

F(ab)′2 fragment goat anti-rabbit is conjugated to Atto 488 NHS and purified by gel permeation chromatography and dialysis to remove unbound dye.

Analysis Note

Unconjugated dye ≤5% of total fluorescence; dye-to protein ratio ≥2

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves