B8756
Bile salts
suitable for microbiology
Synonym(s):
Bile acids sodium salt, cholic acid-deoxycholic acid sodium salt mixture
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About This Item
sterility
non-sterile
Quality Level
form
powder
solubility
distilled water: freely soluble
application(s)
microbiology
storage temp.
10-30°C
General description
Bile salt is an organic sodium salt with a conjugate of any of the bile acids along with either glycine or taurine. Biles salts are composed of four different kinds of bile acids - cholic, deoxycholic, chenodeoxycholic and lithocholic acids.Bile salts are extracted under controlled conditions from purified fresh bile, to be used in bacteriological culture media, where it behaves as a selective inhibitory agent that enables isolation and identification of pathogens. For example, in MacConkey Agar/Broth.
Application
Bile salts may be used as one of the selective agents to prevent the growth of injured Escherichia coli cells, in an experiment done in order to identify the specific agent in the selective media which would inhibit the growth of the injured Escherichia coli ML30 cells. It may also be used for identification of isolates of Vibrio (Beneckea) vulnificus which is a halophilic bacterium, found responsible for causing several human infections.
Other Notes
Mixture of sodium cholate and sodium deoxycholate
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Six derivatives with increased resistance to ox gall (MIC: > or = 1% w/v) and one derivative resistant to sodium cholate (MIC: 0.8% w/v) were obtained from more sensitive original Bifidobacterium strains. These microorganisms, and two additional cholate resistant derivatives
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