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95388

Millipore

Bismuth sulfite Agar

suitable for microbiology, NutriSelect® Basic

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About This Item

UNSPSC Code:
41171606
NACRES:
NA.74

Agency

according to GB 4789.4-2016

Quality Level

100

sterility

non-sterile

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 15 g/L
bismuth ammonium citrate, 1.85 g/L
brilliant green, 0.025 g/L
disodium hydrogen phosphate, 4 g/L
D(+)-glucose, 5 g/L
iron(II) sulfate 7x H2O, 0.55 g/L (equivalentto 0.3g/L iron(ll) sulfate water free)
meat extract, 5 g/L
mixed peptone, 10 g/L
sodium sulfite, 6.15 g/L

manufacturer/tradename

NutriSelect® Basic

technique(s)

microbiological culture: suitable

final pH

7.6±0.2 (25 °C)

application(s)

clinical testing
environmental
food and beverages
microbiology

suitability

selective and differential for Salmonella spp.

Application

Selective medium for the isolation and differentiation of Salmonella typhi and other salmonellae from clinical material etc.
The prepared agar is turbid and greenish-yellow.

Preparation Note

Dissolve 47.55 g in litre distilled water and heat to boiling to dissolve the medium completely. DO NOT STERILIZE. This medium should be prepared, and the plates poured, 24h in advance of their intended use, since the freshly prepared medium is excessively toxic to Salmonellae. It must also be remembered the medium loses selectivity after 3-4 days, even when refrigerated.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Hazard Statements

H412

Precautionary Statements

P273 - P501

Hazard Classifications

Aquatic Chronic 3

Supplementary Hazards

EUH031

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCG3887
BCCF7996
BCCD7618
BCCC4036
BCBS2864V

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Use of a Glucose Bismuth Sulphite Iron Medium for the Isolation of B. typhosus and B. proteus.
W J Wilson et al.
The Journal of hygiene, 26(4), 374-391 (1927-10-01)
J.H. MacCoy
The Journal of Applied Bacteriology, 25, 213-213 (1962)
Further Experience of the Bismuth Sulphite Media in the Isolation of Bacillus typhosus and B. paratyphosus B from Faeces, Sewage and Water.
W J Wilson et al.
The Journal of hygiene, 31(2), 138-161 (1931-04-01)

Articles

Differentiation and Identification Media for Salmonella

Salmonella contamination is the second leading cause of food-borne illness worldwide. Controlling outbreaks of Salmonella is an important task for food regulators, restaurants and the food industry in general. The Salmonella family includes over 2,300 serotypes of bacteria, but two types, Salmonella enteritidis and Salmonella typhimurium, are responsible for about half of all human infections. Most outbreaks of Salmonella are traced back to dairy, poultry and meat products, but Salmonella can grow on nearly any food. Chicken, eggs and their derivative products are particularly high risk.

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