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51463

Millipore

Urea Broth

NutriSelect® Basic, suitable for microbiology

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About This Item

UNSPSC Code:
41171606
NACRES:
NA.74

sterility

non-sterile

Quality Level

100

product line

BioChemika

form

powder

shelf life

limited shelf life, expiry date on the label

composition

dipotassium hydrogen phosphate, 9.5 g/L
phenol red, 0.01 g/L
potassium dihydrogen phosphate, 9.1 g/L
urea, 20 g/L
yeast extract, 0.1 g/L

manufacturer/tradename

NutriSelect® Basic

technique(s)

microbe id | specific enzyme detection: suitable
microbiological culture: suitable

final pH

6.8±0.2 (25 °C)

application(s)

clinical testing
environmental
food and beverages
microbiology

suitability

nonselective and identification for Enterobacter spp.
nonselective and identification for Escherichia coli
nonselective and identification for Klebsiella spp.
nonselective and identification for Proteus spp.
nonselective and identification for Salmonella spp.
nonselective and identification for coliforms
nonselective and identification for enterobacteriaceae

Related Categories

Application

Differential medium for the detection of urea-utilizing microorganisms, acc. to Rustigian and Stuart (1941).

Preparation Note

Dissolve 38.7 g in 1 litre distilled water and filter sterilize. This medium must NOT be heated.

Other Notes

Adaption of urea broth for radiosterilization

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCG8470
BCCF9918
BCCC4038
BCBW4691
BCBT8367

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W.W. Feguson et al.
The Journal of Laboratory and Clinical Medicine, 28, 1715-1715 (1943)
R. Rustigian et al.
Proceedings of the Society For Experimental Biology and Medicine. Society For Experimental Biology and Medicine, 47, 108-108 (1941)
E Eisenberg et al.
The Journal of applied bacteriology, 58(1), 21-25 (1985-01-01)
Urea broth, a medium used for the identification of the genus Proteus, was sterilized by gamma radiation, using radiation doses of 1-1.5 Mrad. The radiation-sterilized medium, modified by adding sodium ascorbate and increasing its phenol red and yeast extract content

Articles

Differentiation of Escherichia coli from coliforms

Sigma-Aldrich.com presents an article concerning Differentiation of Escherichia coli from coliforms.

Differentiation and Identification Media for Salmonella

Salmonella contamination is the second leading cause of food-borne illness worldwide. Controlling outbreaks of Salmonella is an important task for food regulators, restaurants and the food industry in general. The Salmonella family includes over 2,300 serotypes of bacteria, but two types, Salmonella enteritidis and Salmonella typhimurium, are responsible for about half of all human infections. Most outbreaks of Salmonella are traced back to dairy, poultry and meat products, but Salmonella can grow on nearly any food. Chicken, eggs and their derivative products are particularly high risk.

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