MABT1510

Anti-MBP (Citrulline R25) Antibody, clone 1B8

clone 1B8, from mouse

• Synonym(s): Myelin basic protein, Myelin A1 protein, Myelin membrane encephalitogenic protein, Citrullinated MBP
• UNSPSC Code: 12352203
• eCl@ss: 32160702

Properties

• biological source: mouse
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1B8, monoclonal
• species reactivity: human, mouse
• packaging: antibody small pack of 25 μg
• technique(s): ELISA: suitable; electron microscopy: suitable; immunofluorescence: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG1
• NCBI accession no.: NP_001020252
• UniProt accession no.: P02686
• target post-translational modification: citrullination (Arg25)

Description

General description

Myelin basic protein (UniProt: P02686; also known as MBP, Myelin A1 protein, Myelin membrane encephalitogenic protein) is encoded by the MBP gene (Gene ID: 4155) in human. MBP is a homodimeric protein that is found in both the central and the peripheral nervous system. It is one of the most abundant protein component of the myelin membrane in the CNS and plays a role in both the formation and stabilization of this myelin membrane. It is responsible for adhesion of the cytosolic surfaces of multilayered compact myelin. MBP can interact with many polyanionic proteins including actin, tubulin, calmodulin, and clathrin, and with negatively charged lipids. MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system. MBP expression begins abruptly in 14-16-week-old fetuses. Even smaller isoforms seem to be produced during embryogenesis; some of these persisting in the adult. MBP citrullination by peptidylarginine deiminase (PAD) leads to incomplete protein-lipid bilayer interactions and increases its vulnerability to proteolytic enzymes, resulting in disorganization of the myelin sheath in the central nervous system. Citrullinated MBP (citMBP) has been suggested to be a hallmark of demyelination. MBP citrullination has frequently been demonstrated in various CNS disorders. citMBP at R25, R122, and R130 is reported in the brains of subjects with Creutzfeldt-Jakob disease (CJD). Clone 1B8 specifically recognizes citMBP at R25. (Ref.: Jang, B., et al. (2017). Mol. Neurobiol. 55(4); 3172-3184; Boggs, JM (2006). Cell. Mol. Life Sci. 63(17); 1945-1961).

Specificity

Clone 1B8 is a mouse monoclonal antibody that detects human and murine myelin basic protein citrullinated on residue 25. It targets an epitope with in 18 amino acids from the N-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 18 amino acids from isoform 5 of human Myelin Basic Protein with citrullination on residue 25.

Application

Anti-MBP (Citrulline R25), clone 1B8, Cat. No. MABT1510, is a mouse monoclonal antibody that detects is Myelin basic protein citrullinated on residue 25 and has been tested for use in ELISA, Electron Microscopy, Immunofluorescence, Immunohistochemistry, and Western Blotting.

Immunohistochemistry Analysis: A representative lot detected MBP (Citrulline R25) in Immunohistochemistry applications (Jang, B., et. al. (2018). Mol Neurobiol. 55(4):3172-3184).

Electron Microscopy Analysis: A representative lot detected MBP (Citrulline R25) in Electron Microscopy applications (Jang, B., et. al. (2018). Mol Neurobiol. 55(4):3172-3184).

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected MBP (Citrulline R25) in Myelin basic protein (MBP) treated with human PAD2 (Courtesy of Byungki Jang, Ph.D./Eun-Kyoung Choi, Ph.D., Ilsong Institute of Life Science, Hallym University, South Korea).

Immunofluorescence Analysis: A representative lot detected MBP (Citrulline R25) in Immunofluorescence applications (Jang, B., et. al. (2018). Mol Neurobiol. 55(4):3172-3184).

Western Blotting Analysis: A representative lot detected MBP (Citrulline R25) in Western Blotting applications (Jang, B., et. al. (2018). Mol Neurobiol. 55(4):3172-3184).

ELISA Analysis: A representative lot detected MBP (Citrulline R25) in ELISA applications (Jang, B., et. al. (2018). Mol Neurobiol. 55(4):3172-3184).

Research Category
Cell Structure

Quality

Evaluated by Western Blotting in Myelin basic protein (MBP) either treated with human PAD-2.

Western Blotting Analysis: 1 µg/mL of this antibody detected Myelin basic protein (MBP) treated with human human Peptidyl Arginine Deiminase 2 (PAD-2) (200 ng for 4 hours at 37°C) with R25 citrullination.

Target description

~19 kDa observed; 18.59 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.