MABT1443
Anti-phospho-Connexin 43 (Ser368) Antibody, clone 2C6
Synonym(s):
Gap junction alpha-1 protein;Connexin-43;Cx43;Gap junction 43 kDa heart protein
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About This Item
UNSPSC Code:
12352203
Recommended Products
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
2C6, monoclonal
mol wt
calculated mol wt 43.01 kDa
observed mol wt ~42 kDa
purified by
using protein G
species reactivity
rat
species reactivity (predicted by homology)
monkey, bovine, porcine, canine, human, feline
packaging
antibody small pack of 100 μL
technique(s)
western blot: suitable
isotype
IgG2aκ
epitope sequence
C-terminal
Protein ID accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
phosphorylation (pSer368)
Gene Information
human ... GJA1(2697)
General description
Gap junction alpha-1 protein (UniProt: P17302; also known as Connexin-43, Cx43, Gap junction 43 kDa heart protein) is encoded by the GJA1 (also known as GJAL) gene (Gene ID: 2697) in human. Cx43 is a member of the gap junction family, which is composed of intercellular channels allowing direct cell-to-cell communication between neighboring cells via the exchange of small ions and metabolites. It is a multi-pass membrane protein with three cytoplasmic domains, four transmembrane domains, and two extracellular domains. It is one of the most widely studied gap junction proteins that regulates N-cadherin expression during collective cell migration. It also acts as a negative regulator of bladder capacity by enhancing intercellular electrical and chemical transmission and thereby exsensitizing bladder muscles to cholinergic neural stimuli and causing them to contract. It is also expressed in gap junctions of the cardiac ventricle and plays an essential role in gap junction communication in the ventricles. Cx43 can be phosphorylated at multiple sites and phosphorylation is shown to regulate the kinetics of its trafficking, assembly, gating and turnover in a cell-cycle-stage specific manner. Cx43 is phosphorylated at Ser-368 by PKCg (PRKCG) and this phosphorylation is reported to induce disassembly of gap junction plaques and inhibition of gap junction activity and triggers its internalization into small vesicles leading to proteasome-mediated degradation. (Ref.: Solan, JL., and Lampe, PD. (2020). Biomolecules. 10(12); 1596; Kotini, M., et al. (2018). Nature Commun. 9; article 3846).
Specificity
Clone 2C6 is a mouse monoclonal antibody that detects Connexin 43 phosphorylated on serine 368.
Immunogen
KLH-conjugated linear peptide corresponding to 11 amino acids surrounding phosphoserine 368 from the C-terminal, cytoplasmic domain of human Connexin 43.
Application
Quality Control Testing
Evaluated by Western Blotting in NRK cell lysate.
Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected Connexin 43 phosphorylated on serine 368 in lysates from untreated NRK cells and in NRK cells treated with 12-O-tetradecanoylphorbol-13-acetate (TPA; 25 nM; 40 min).
Tested Applications
Western Blotting Analysis (WB): A representative lot of this antibody detected phospho-Connexin-43 (Ser368) in NRK cells. ( Solan, JL., and Lampe, PD. (2020). Biomolecules. 10(12); 1596).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in NRK cell lysate.
Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected Connexin 43 phosphorylated on serine 368 in lysates from untreated NRK cells and in NRK cells treated with 12-O-tetradecanoylphorbol-13-acetate (TPA; 25 nM; 40 min).
Tested Applications
Western Blotting Analysis (WB): A representative lot of this antibody detected phospho-Connexin-43 (Ser368) in NRK cells. ( Solan, JL., and Lampe, PD. (2020). Biomolecules. 10(12); 1596).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-phospho-Connexin 43 (Ser368), clone 2C6, Cat. No. MABT1443, is a mouse monoclonal antibody that detects Connexin-43 phosphorylated on serine 368 and is tested for use in Western Blotting.
Physical form
Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Recommended storage: +2°C to +8°C.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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