AP132P
Goat Anti-Rabbit IgG Antibody, Peroxidase Conjugated
1 mg/mL (after reconstitution), Chemicon®
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.46
biological source
goat
Quality Level
conjugate
peroxidase conjugate
antibody form
affinity purified immunoglobulin
antibody product type
secondary antibodies
clone
polyclonal
species reactivity
rabbit
manufacturer/tradename
Chemicon®
concentration
1 mg/mL (after reconstitution)
technique(s)
ELISA: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG
shipped in
wet ice
target post-translational modification
unmodified
General description
Affinity purified goat anti-rabbit, HRP conjugated.
Specificity
Specific for rabbit IgG, heavy and light chain.
Application
ELISA and Western Blots:
1:5,000-1:100,000 dilution can be used.
Immunohistochemistry:
1:500-1:5,000 dilution can be used.
Optimal working dilutions must be determined by end user.
1:5,000-1:100,000 dilution can be used.
Immunohistochemistry:
1:500-1:5,000 dilution can be used.
Optimal working dilutions must be determined by end user.
Research Category
Secondary & Control Antibodies
Secondary & Control Antibodies
Research Sub Category
Whole Immunoglobulin Secondary Antibodies
Whole Immunoglobulin Secondary Antibodies
This Goat anti-Rabbit IgG Antibody, Peroxidase Conjugated is validated for use in ELISA, IH, WB for the detection of Rabbit IgG.
Physical form
ImmunoAffinity Purified
Purified goat IgG conjugated to horseradish peroxidase in buffer containing 0.01M PBS, pH 7.1, with 15mg/mL BSA. Lyophilized.
RECONSTITUTION:
Reconstitute with sterile distilled water to match the volume indicated on the vial label.
RECONSTITUTION:
Reconstitute with sterile distilled water to match the volume indicated on the vial label.
Storage and Stability
Maintain lyophilized product at 2-8°C for up to 12 months from date of shipment.
After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.
WARNING: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of HRP.
After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.
WARNING: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of HRP.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
13 - Non Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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