AC113

Anti-Neomycin Phosphotransferase II Antibody | AC113

from rabbit, purified by affinity chromatography

• Synonym(s): Aminoglycoside 3′-phosphotransferase, APH(3′)-II, APH(3′)II, Kanamycin kinase, type II, Neomycin-kanamycin phosphotransferase type II
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human
• technique(s): western blot: suitable
• NCBI accession no.: YP_788126
• UniProt accession no.: P00552
• shipped in: wet ice
• target post-translational modification: unmodified

Description

General description

Neomycin phosphotransferase II confers resistance to kanamycin and neomycin in bacteria and G418 (Geneticin^®, G418 Sulfate) in mammalian cells. These antibiotics bind to ribosomal components and inhibit protein synthesis. NPT II inhibits these antibiotics through phosphorylation, which is thought to interfere with their active transport into the cell. Immunoblot analysis of transfected cell lysates with anti NPT II can be used to gauge relative transfection efficiencies in experiments where multiple transfections are performed in parallel with different plasmids.

Specificity

This antibody reacts to lysates from cells transfected or transformed with a plasmid containing a kanamycin/neomycin resistance gene, nptII (Aminoglycoside 3′-phosphotransferase).

Immunogen

Recombinant protein corresponding to full length neomycin phosphotransferase (NPT) II.

Application

Research Category
Secondary & Control Antibodies

Research Sub Category
Kinases & Phosphatases

Quality

Evaluated by Western Blot in untreated and neomycin treated HEK293 cells transfected with pUSEamp(-).

Western Blot Analysis: A 1:1,000 dilution of this antibody detected Neomycin phosphotransferase II in 10 µg of neomycin treated HEK293 cells transfected with pUSEamp(-).

Target description

~29 kDa observed.

Physical form

Affinity purified

Hek 293 +/- transfection with pUSEamp(-)and neomycin treatment.

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Untreated and neomycin treated HEK293 cells transfected with pUSEamp(-).

Legal Information

Geneticin is a registered trademark of Gibco BRL Life Technologies, Inc.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(C): Not applicable