AB5541
Anti-Glial Fibrillary Acidic Protein Antibody
CHEMICON®, chicken polyclonal
Synonym(s):
GFAP
Sign Into View Organizational & Contract Pricing
All Photos(7)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
product name
Anti-Glial Fibrillary Acidic Protein Antibody, Chemicon®, from chicken
biological source
chicken
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human, mouse, rat, bovine, pig
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... GFAP(2670)
General description
Glial fibrillary acidic protein (GFAP) is a class-III intermediate filament. Type III intermediate filaments contain three domains, the most conserved of which is the rod domain. The specific sequence for this region of the protein may differ between the different intermediate filament genes for type III proteins, but the structure of the protein is highly conserved. GFAP is the main constituent of intermediate filaments in astrocytes and serves as a cell specific marker that distinguishes differentiated astrocytes from other glial cells during the development of the central nervous system. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes.
Specificity
Expected to cross-react with bovine and porcine.
Glial fibrillary acidic protein (GFAP). Reacts with both native and recombinant protein.
Other species have not yet been tested.
Immunogen
Purified bovine GFAP.
Application
Immunohistochemistry(paraffin):
Representative images from a previous lot. Pictures of Optimal Staining With Citrate Buffer Epitope Retrieval: Human Brain.
Immunocytochemistry:
1:200-1:1,000 on cells in tissue culture fixed for one minute in 3.7% formalin and one minute in -20°C methanol.
Western blot:
1:100-1:500 using ECL. Recognizes a band of 55 kDa. May also react with a band of 50 kDa.
Immunohistochemistry:
1:100-1:500 on frozen tissue sections. Suggested fixative is 4% paraformaldehyde.
Optimal working dilutions must be determined by the end user.
STAINING PATTERN:
The antibody stains sharply defined cytoplasmic filaments of astroglia cells in tissue culture. Stains processes of astrocytes in sections of brain tissues.
Representative images from a previous lot. Pictures of Optimal Staining With Citrate Buffer Epitope Retrieval: Human Brain.
Immunocytochemistry:
1:200-1:1,000 on cells in tissue culture fixed for one minute in 3.7% formalin and one minute in -20°C methanol.
Western blot:
1:100-1:500 using ECL. Recognizes a band of 55 kDa. May also react with a band of 50 kDa.
Immunohistochemistry:
1:100-1:500 on frozen tissue sections. Suggested fixative is 4% paraformaldehyde.
Optimal working dilutions must be determined by the end user.
STAINING PATTERN:
The antibody stains sharply defined cytoplasmic filaments of astroglia cells in tissue culture. Stains processes of astrocytes in sections of brain tissues.
This Anti-Glial Fibrillary Acidic Protein Antibody is validated for use in IC, IH, IH(P), WB for the detection of GFAP.
Quality
Routinely evaluated by Western Blot on Mouse Brain lysates.
Western Blot Analysis: 1:1000 dilution of this lot detected glial fibrillary acidic protein on 10 μg of Mouse Brain lysates.
Western Blot Analysis: 1:1000 dilution of this lot detected glial fibrillary acidic protein on 10 μg of Mouse Brain lysates.
Target description
55 kDa
Linkage
Replaces: 04-1031; 04-1062
Physical form
Purified chicken polyclonal in buffer containing PBS containing 0.02% sodium azide.
Storage and Stability
Stable for 1 year at -20°C in undiluted aliquots from date of receipt. Do not store in a self-defrosting freezer.
Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
Astrocytes, astrocytomas, neural stem cells.
Astrocytes, astrocytomas, neural stem cells.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Not finding the right product?
Try our Product Selector Tool.
recommended
Product No.
Description
Pricing
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Tanycyte-like cells form a blood-cerebrospinal fluid barrier in the circumventricular organs of the mouse brain.
Langlet, F; Mullier, A; Bouret, SG; Prevot, V; Dehouck, B
The Journal of Comparative Neurology null
In vivo MRI analysis of an inflammatory injury in the developing brain.
Lodygensky, GA; West, T; Stump, M; Holtzman, DM; Inder, TE; Neil, JJ
Brain, behavior, and immunity null
Diffusion characteristics associated with neuronal injury and glial activation following hypoxia-ischemia in the immature brain.
Lodygensky, GA; West, T; Moravec, MD; Back, SA; Dikranian, K; Holtzman, DM; Neil, JJ
Magn. Reson. in Med. null
Increased atypical PKC expression and activity in the phrenic motor nucleus following cervical spinal injury.
Guenther, CH; Windelborn, JA; Tubon, TC; Yin, JC; Mitchell, GS
Experimental neurology null
Taurine in drinking water recovers learning and memory in the adult APP/PS1 mouse model of Alzheimer's disease.
Kim, HY; Kim, HV; Yoon, JH; Kang, BR; Cho, SM; Lee, S; Kim, JY; Kim, JW; Cho, Y; Woo, J; Kim, Y
Scientific Reports null
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service