361565

GSK3β Inhibitor XIX, IM-12

The GSK3β Inhibitor XIX, IM-12 controls the biological activity of GSK3β. This small molecule/inhibitor is primarily used for Phosphorylation & Dephosphorylation applications.

• Synonym(s): GSK3β Inhibitor XIX, IM-12, IM-12
• Empirical Formula (Hill Notation): C₂₂H₂₀FN₃O₂
• Molecular Weight: 377.41
• UNSPSC Code: 12352200

Properties

• Quality Level: 100
• Assay: ≥95% (HPLC)
• form: powder
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; protect from light
• color: orange-yellow
• solubility: DMSO: 100 mg/mL; DMSO: soluble
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

A cell-permeable indolylmaleimide derivative and SB-216763 analog that acts as a GSK-3β inhibitor, with an IC₅₀ of 53 nM in an in vitro binding assay, compared with 92 nM for SB-216763 in the same assay condition, and reduces GSK-3β activity to 27% in an enzymatic assay at 3 µM concentration. It is shown to attenuate hNPC (human neural progenitor cells) cell proliferation, and enrich β-catenin around the nucleus of ST14 cells in the same extent of SB-216763. Furthermore, it can mimic the effect of stabilized β-catenin, up-regulates TCF-activity in a luciferase reporter gene assay, and enhances human progenitor cell differentiation.

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Schmole, AC., et al. 2010. Bioorg. Med. Chem.18, 6785.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable