07-449-AF488

Anti-trimethyl-Histone H3 (Lys27) Antibody, Alexa Fluor^™ 488 Conjugate

from rabbit, ALEXA FLUOR^™ 488

• Synonym(s): Anti-H3.4, Anti-H3/g, Anti-H3FT, Anti-H3t, Anti-HIST3H3, Histone H3.1t, H3/t, H3t, H3/g, trimethyl-Histone (Lys27), Alexa Fluor^™ 488 Conjugate
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• conjugate: ALEXA FLUOR^™ 488
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: rat, human, mouse
• technique(s): immunocytochemistry: suitable
• isotype: IgG
• NCBI accession no.: NM_003493
• UniProt accession no.: Q16695
• shipped in: wet ice
• target post-translational modification: trimethylation (Lys27 )
• Gene Information: human ... H3C1(8350) ; mouse ... H3C1(360198) ; rat ... H3C1(679994)

Description

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone H3 variants (H3.1, H3.2 and H3.3) have been implicated in the epigenetic memory of cellular state. Genome-wide patterns of H3 are dependent on amino acid sequence and change with cellular differentiation at developmentally regulated loci.

Specificity

Broad species cross-reactivity expected.

H3K27me3 specificity of the unconjugated antibody (Cat. No. 07-449) has been confirmed using the Absurance Histone H3 Antibody Specificity Array (Cat. No. 16-667) and the Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665).

Immunogen

Epitope: H3K27me3

KLH-conjugated, synthetic 2X-branched peptide containing the sequence …AR(Kme3)SAP… in which Kme3 corresponds to trimethyl-lysine at residue 27 of human histone H3.

Application

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

The unconjugated antibody (Cat. No. 07-449) is shown to be suitable also for immunohistochemistry, immunocytochemistry, immunoprecipitation, multiplex assay, Western blotting, chromatin immunoprecipitation (ChIP), and ChIP-seq applications.

This Anti-trimethyl-Histone H3 (Lys27) Antibody, Alexa Fluor^™ 488 Conjugate is validated for use in Immunocytochemistry for the detection of trimethyl-Histone H3.

Quality

Evaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected trimethyl-Histone H3 (Lys27) in A431 cells.

Target description

~16 kDa observed

Physical form

Protein A purified

Purified rabbit antibody conjugate in PBS with 15 mg/ml BSA and 0.1% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2