850185P

08:0 PI(4,5)P2

Avanti Polar Lipids

• Synonym(s): PIP2[4′,5′](8:0/8:0); 110665
• Empirical Formula (Hill Notation): C₂₅H₅₈N₃O₁₉P₃
• CAS Number: 852043-37-9
• Molecular Weight: 797.66
• UNSPSC Code: 51191904
• NACRES: NA.25

Properties

• description: 1,2-dioctanoyl-sn-glycero-3-phospho-(1′-myo-inositol-4′,5′-bisphosphate) (ammonium salt)
• Assay: >99% (TLC)
• form: powder
• packaging: pkg of 1 × 100 μg (with stopper and crimp cap (850185P-100ug)); pkg of 1 × 500 μg (with stopper and crimp cap (850185P-500ug))
• manufacturer/tradename: Avanti Polar Lipids
• lipid type: cardiolipins; phospholipids
• shipped in: dry ice
• storage temp.: −20°C
• SMILES string: [H][C@@](COP([O-])(O[C@H]1[C@H](O)[C@@H](OP(O)([O-])=O)[C@H](OP([O-])(O)=O)[C@@H](O)[C@H]1O)=O)(OC(CCCCCCC)=O)COC(CCCCCCC)=O.[NH4+].[NH4+].[NH4+]

Description

General description

Although PI(4,5)P2 is a minor component of cell membranes, it plays a critical role as a substrate for a number of important signaling proteins. PI(4,5)P2 is an intermediate in the IP3/DAG pathway where it is hydrolyzed by phospholipase C to liberate the second messengers, inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG). PI(4,5)P2 is also a substrate for PI 3-kinase where it is phosphorylated to PI(3,4,5)P3, an activator of downstream signaling components such as the protein kinase AKT. The dioctanoyl derivative of PI(4,5)P2 is more water soluble than its long chain counterparts.

Application

08:0 PI(4,5)P2 is suitable for use to identify its binding site in transient receptor potential vanilloid 4 (TRPV4 N) terminus. It has been used in in vitro binding assay to determine the binding of G-protein coupled receptor kinases (GRKs) and β2-adrenergic receptor (β₂AR).

Packaging

2 mL Amber Serum Vial with Stopper and Crimp Cap (850185P-100ug)

2 mL Amber Serum Vial with Stopper and Crimp Cap (850185P-500ug)

Legal Information

Avanti Research is a trademark of Avanti Polar Lipids, LLC

Safety Information

• Storage Class Code: 11 - Combustible Solids