Skip to Content
Merck
All Photos(1)

Key Documents

927538

Sigma-Aldrich

AlkFAA-alkyne

≥95%

Synonym(s):

2-Fluoro-N-(hex-5-yn-1-yl)acrylamide

Sign Into View Organizational & Contract Pricing


About This Item

Empirical Formula (Hill Notation):
C9H12FNO
Molecular Weight:
169.20
UNSPSC Code:
12352101
NACRES:
NA.21

Quality Level

Assay

≥95%

form

semisolid

storage temp.

−20°C

SMILES string

O=C(NCCCCC#C)C(F)=C

Application

AlkFAA-alkyne is a Michael acceptor probe that was designed to label cysteines, but is not very effective. The compound can be used as a negative control when run with other cysteine-reactive probes. A method was developed using cysteine-reactive compounds to allow for unbiased analysis of proteomic data in quantitative applications . The method uses light or heavy labeling with the isotopically labelled desthiobiotin azide (isoDTB) tag for mass spectrometry analysis . Analysis then uses the isotopic tandem orthogonal proteolysis activity-based protein profiling (isoTOP-ABPP) workflow

related product

Product No.
Description
Pricing

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Patrick R A Zanon et al.
Angewandte Chemie (International ed. in English), 59(7), 2829-2836 (2019-11-30)
Rapid development of bacterial resistance has led to an urgent need to find new druggable targets for antibiotics. In this context, residue-specific chemoproteomic approaches enable proteome-wide identification of binding sites for covalent inhibitors. Described here are easily synthesized isotopically labeled
Eranthie Weerapana et al.
Nature, 468(7325), 790-795 (2010-11-19)
Cysteine is the most intrinsically nucleophilic amino acid in proteins, where its reactivity is tuned to perform diverse biochemical functions. The absence of a consensus sequence that defines functional cysteines in proteins has hindered their discovery and characterization. Here we

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service