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UHPLC Analysis of Cannabinoids on Ascentis Express C18

UHPLC Analysis of Cannabinoids on Ascentis Express C18

Figure 1.

Conditions

InstrumentShimadzu Nexera X2
ColumnAscentis Express C18, 15 cm x 3.0 mm I.D., 2.7 µm 
Mobile Phase[A] water (0.1% formic acid); [B] acetonitrile (0.085% formic acid) 
Gradient70% B to 88% B in 6 min 
Flow Rate1.0 mL/min 
Pressure5075 psi 
Column Temp.30 °C
DetectorUV, 220 nm
Injection0.6 µL
SampleMixture of 14 cannabinoids, 0.04 mg/mL, 50:50 water:acetonitrile
Table 1

Description

Analysis NoteAs the popularity of cannabis-infused products increases, there is a growing need to characterize the type and content of the cannabinoids found in the product. This application demonstrates the ability of the Ascentis Express C18 column to baseline resolve 14 structurally-similar cannabinoids, in under seven minutes, with excellent peak shape.
Legal InformationAscentis is a trademark of Sigma-Aldrich Co. LLC
Snap-N-Spike is a trademark of Cerilliant Corporation
Snap-N-Shoot Standards is a trademark of Cerilliant Corporation
Application NoMUR110618A
Table 2
Materials
Product No.Product NameDescriptionPricing
53816-UAscentis® Express C18, 2.7 μm HPLC Column2.7 μm particle size, L × I.D. 15 cm × 3 mm
1.15333Waterfor chromatography (LC-MS Grade) LiChrosolv®
1.00029Acetonitrilehypergrade for LC-MS LiChrosolv®
5.33002Formic acid 98% - 100%for LC-MS LiChropur
C-152Cannabidivarinic Acid (CBDVA) solution1.0 mg/mL in acetonitrile, certified reference material, ampule of 1 mL, Cerilliant®
C-140Cannabidivarin solution1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®
C-144Cannabidiolic acid solution1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®
C-142Cannabigerolic acid solution1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®
C-141Cannabigerol solution1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®
T-108THC Cannabinoids Mixture-3 solution1.0 mg/mL in methanol (each analyte), certified reference material, ampule of 0.5 mL, Cerilliant®
T-094Tetrahydrocannabivarin (THCV) solution1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®
T-032(−)-Δ8-THC solution1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®
C-154(±)-Cannabicyclol (CBL) solution1.0 mg/mL in acetonitrile, certified reference material, ampule of 1 mL, Cerilliant®
C-143Cannabichromene solution1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®
T-093Delta9-Tetrahydrocannabinolic acid A solution1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®
C-150Cannabichromenic Acid (CBCA) solution1.0 mg/mL in acetonitrile, certified reference material, ampule of 1 mL, Cerilliant®
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