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G1006878

HPLC Analysis of Cetirizine and Hydroxyzine on Ascentis® Express F5

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: Ascentis Express F5, 10 cm x 3.0 mm I.D., 2.7 μm particles (53578-U)
mobile phase: 5 mM ammonium formate pH 3.0 with formic acid in 90:10, acetonitrile:water
flow rate: 0.6 mL/min
pressure: 1300 psi (90 bar)
column temp.: 25 °C
detector: UV, 254 nm
injection: 2 μL
sample: 50 μg/mL in 90:10, acetonitrile:water

suitability

application for HPLC

application(s)

pharmaceutical (small molecule)

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Cetirizine dihydrochloride solution, 1.0 mg/mL in acetonitrile: water (9:1) (as free base), ampule of 1 mL, certified reference material, Cerilliant®
    Hydroxyzine dihydrochloride solution, 1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®

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