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G005445

HPLC Analysis of Milk Thistle Standards on Ascentis® Express C18

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: Ascentis Express C18, 10 cm x 3 mm I.D., 2.7 μm particles (53814-U)
mobile phase: [A] water with 0.1% formic acid; [B] methanol
gradient: held at 35% B for 3 min; 35 to 45% B in 10 min; held at 45% B for 2 min; 45 to 100% B in 5 min
flow rate: 0.6 mL/min
pressure: 4105 psi (283 bar)
column temp.: 35 °C
detector: UV, 254 nm
injection: 20 μL
sample: 20 μg/mL in 80:20, water:methanol

suitability

application for HPLC

application(s)

vitamins, nutraceuticals, and natural products

Analysis Note

This application demonstrates the suitability of Ascentis Express C18 for the efficient separation of milk thistle related compounds.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

analytical column

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