G003761

HPLC Analysis of Amphetamines in Urine on Discovery^® HS F5 after SPE using Discovery^® DSC-MCAX and Standard C18

application for SPE, application for HPLC

Properties

• technique(s): HPLC: suitable; solid phase extraction (SPE): suitable
• test parameters: sample preparation: SPE (Solid Phase Extraction); sample/matrix: human urine spiked amphetamine and methylamphetamine at with 2 mg/mL; SPE tube/cartridge: Discovery DSC-MCAX, 100 mg/3mL (52783-U); condition: DSC-MCAX SPE tube: 1 mL methanol; 1 mL 50 mM ammonium acetate, pH 6.0; C-18 SPE tube: 1 mL methanol; 1 mL DI water; sample addition: 1 mL; washing: DSC-MCAX SPE tube: 1 mL 50 mM ammonium acetate, pH 6.0; 1 mL 1M acetic acid; 1mL methanol; C-18 SPE tube: 1 mL DI water; 1 mL 20% methanol; elution: DSC-MCAX SPE tube: 1 mL 5% ammonium hydroxide in methanol; C-18 SPE tube: 1 mL methanol; eluate post-treatment: evaporate to dryness with nitrogen at room temperature, reconstitute in mobile phase; column: Discovery HS F5, 15 cm x 4.6 mm I.D., 5 μm particles (567516-U); mobile phase: [A] 10 mM ammonium acetate, pH 4.5: [B] methanol; (35:65, A:B); flow rate: 2 mL/min; column temp.: 40 °C; detector: UV, 210 nm; injection: 10 μL
• suitability: application for HPLC; application for SPE
• application(s): forensics and toxicology

Description

Application

Sample Pre-Treatment:
1 mL human urine was spiked with 2 mg/mL amphetamine and methylamphetamine. The spiked sample was diluted 1:1 with 1:1 with 50 mM ammonium acetate, pH 6.0.

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany