V9134

Monoclonal Anti-Vascular Endothelial Growth Factor Receptor-2 antibody produced in mouse

clone KDR-1, ascites fluid

• Synonym(s): Anti-KDR, Anti-VEGF R-2
• MDL number: MFCD01634355
• UNSPSC Code: 51111800
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: KDR-1, monoclonal
• contains: 15 mM sodium azide
• species reactivity: human
• technique(s): immunohistochemistry (frozen sections): 1:800 using human placenta; indirect ELISA: suitable
• isotype: IgG1
• UniProt accession no.: P35968
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... KDR(3791)

Description

General description

Monoclonal Anti-Vascular Endothelial Growth Factor Receptor-2 (mouse IgG1 isotype) is derived from the KDR-1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Vascular endothelial growth factor (VEGF), is a homodimeric heparin-binding glycoprotein. It is a member of a family of endothelial cell mitogenic and angiogenic factors. The VEGFR2 gene has been mapped to human chromosome 4q11-q12, which is the same locus for PDGF receptor and c-kit.

The antibody reacts specifically with VEGF receptor 2 (KDR) and does not recognize VEGF receptor-1 (Flt1 receptor), VEGF receptor 3 (sFlt4), and PDGF-Rβ.

Immunogen

recombinant human extracellular domain of VEGFR-2 (KDR).

Application

Monoclonal Anti-Vascular Endothelial Growth Factor Receptor-2 antibody produced in mouse has been used in:

• immunohistochemistry
• histology
• immunostaining
• enzyme linked immunosorbent assay (ELISA)

Monoclonal Anti-Vascular Endothelial Growth Factor Receptor-2 antibody produced in mouse was used for FACS analysis of HUVECs.

Biochem/physiol Actions

Vascular endothelial growth factor (VEGF) specifically stimulates the proliferation of endothelial cells isolated from both small and large vessels. These include endothelial cells from adrenal cortex, cerebral cortex, fetal and adult aorta and human umbilical vein.

Vascular endothelial growth factors receptors (VEGF Rs) are receptor tyrosine kinases that bind to VEGF. VEGF plays an important role in embryonic vasculogenesis, angiogenesis and homeostasis. The binding of VEGF to VEGF R2 results in activation of MAPK, PI3K, PKC, FAK and Src kinase pathways. The biological roles of VEGF R2 include in epithelial cell growth, proliferation, survival, migration, vascular permeability and angiogenesis.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable