T7927

L-Tyrosine Decarboxylase Apoenzyme from Streptococcus faecalis

<0.005 unit/mg solid (without pyridoxal 5-phosphate), ≥0.05 unit/mg solid (with excess pyridoxal 5-phosphate)

• Synonym(s): L-(−)-Tyrosine Apodecarboxylase, L-Tyrosine Carboxy-lase apoenzyme, TAD
• CAS Number: 9002-09-9
• Enzyme Commission number: 4.1.1.25 (BRENDA, IUBMB)
• EC Number: 232-652-9
• MDL number: MFCD00131472
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: Streptococcus faecalis
• Quality Level: 200
• form: powder
• specific activity: ≥0.05 unit/mg solid (with excess pyridoxal 5-phosphate); <0.005 unit/mg solid (without pyridoxal 5-phosphate)
• storage temp.: −20°C

Description

Application

L-Tyrosine decarboxylase apoenzyme from Streptococcus faecalis has been used:

• in a study to purify and characterize tyrosine decarboxylase and aromatic-L-amino-acid decarboxylase
• in a study to investigate the stereospecificity of sodium borohydride reduction of tyrosine decarboxylase
• in protein synthesis studies

Biochem/physiol Actions

L-Tyrosine Decarboxylase Apoenzyme is an aromatic L-amino acid decarboxylase. The enzyme activity of tyrosine decarboxylase (TDC) is dependent on the cofactor pyridoxal 5-phosphate (PLP). It catalyzes the decarboxylation of dihydroxyphenylalanine.

Other Notes

Dried cells grown on vitamin B₆-deficient medium.

Unit Definition

One unit will liberate 1.0 μmole of CO₂ from L-tyrosine per min at pH 5.5 at 37 °C.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves