SRE0008

Galactose-1-phosphate Uridyltransferase from galactose-adapted yeast

Suitable for manufacturing of diagnostic kits and reagents, lyophilized powder, 20-60 units/mg protein (modified Warburg-Christian)

• Synonym(s): GALT, UDP glucose:α-D-galactose-1-phosphate uridyltransferase
• CAS Number: 9016-11-9
• Enzyme Commission number: 2.7.7.12 (BRENDA, IUBMB)
• MDL number: MFCD00131120
• UNSPSC Code: 12352204
• NACRES: NA.32

Properties

• form: lyophilized powder
• specific activity: 20-60 units/mg protein (modified Warburg-Christian)
• composition: Protein, 15-35%
• application(s): diagnostic assay manufacturing
• foreign activity: 6-phosphogluconate dehydrogenase ≤0.5%; UDP glucose pyrophosphorylase and galactokinase ≤0.2%
• shipped in: wet ice
• storage temp.: −20°C

Description

Biochem/physiol Actions

Galactose-1-phosphate uridyltransferase catalyzes the conversion of UDP-glucose to galactose-1-phosphate and galactose-1-phosphate to UDP-glucose.

Unit Definition

One unit will form 1.0 μmole of glucose 1-phosphate from UDP-glucose, galactose 1-phosphate and NADP⁺ per min at pH 8.7 at 25 °C as detected by a coupled system using phosphoglucomutase.

Physical form

Contains buffer salts as citrate and reduced glutathione

Analysis Note

Protein determined by modified Warburg-Christian

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable