Skip to Content
Merck
All Photos(3)

Key Documents

More Documents

SAB4200734

Sigma-Aldrich

Anti-IL6 high affinity antibody, Mouse monoclonal

clone IL6-2A3, purified from hybridoma cell culture

Synonym(s):

Anti-B-cell stimulatory factor 2 (BSF-2), Anti-CTL differentiation factor (CDF), Anti-Hybridoma growth factor, Anti-Interferon beta-2 (IFN-beta-2), Anti-Interleukin-6

Sign Into View Organizational & Contract Pricing
All Photos(3)

About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

200

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

IL6-2A3, monoclonal

form

buffered aqueous solution

species reactivity

human

concentration

~1.0 mg/mL

technique(s)

ELISA: suitable
immunoblotting: 0.125-0.25 μg/mL using recombinant human IL6 expressed in HEK-293T cells
immunoprecipitation (IP): 10-20 μg/test using concentrated supernatant of human osteosarcoma MG-63 cells treated with IL1β

isotype

IgG2a

UniProt accession no.

P05231

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... IL6(3569)

General description

Interleukin-6 (IL6) is a multifunctional cytokine.

Immunogen

recombinant human IL6

Application

Anti-IL6 high affinity antibody, Mouse monoclonal has been used in
  • immunoblotting
  • immunoprecipitation
  • ELISA

Biochem/physiol Actions

Interleukin-6 (IL6) plays a wide variety of important biological roles in the immune, nervous and endocrine system. It is an essential component for hybridoma growth and is found in many supplemental cloning media. IL6 regulates acute and chronic inflammatory diseases and has been proven to be an excellent target for clinical treatment against autoimmune disorders such as rheumatoid arthritis, juvenile idiopathic arthritis and Castleman′s disease. IL6 also plays a key role in the pathogenesis of multiple myeloma.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Not finding the right product?  

Try our Product Selector Tool.

recommended

Product No.
Description
Pricing

Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

A phase 2 multicentre study of siltuximab, an anti-interleukin-6 monoclonal antibody, in patients with relapsed or refractory multiple myeloma
Voorhees PM, et al.
British Journal of Haematology, 161(3), 357-366 (2013)
IL-6 in autoimmune disease and chronic inflammatory proliferative disease
Ishihara K and Hirano T
Cytokine & Growth Factor Reviews, 13(4-5), 357-368 (2002)
L M Biasucci et al.
Circulation, 94(5), 874-877 (1996-09-01)
Elevated plasma levels of C-reactive protein have been found in the majority of patients with unstable angina. The evidence of elevated levels of acute-phase proteins in unstable angina is in line with a growing body of evidence that suggests that
Peter M Voorhees et al.
British journal of haematology, 161(3), 357-366 (2013-02-26)
Interleukin-6 (IL6) plays a central role in multiple myeloma pathogenesis and confers resistance to corticosteroid-induced apoptosis. We therefore evaluated the efficacy and safety of siltuximab, an anti-IL6 monoclonal antibody, alone and in combination with dexamethasone, for patients with relapsed or
Luca Semerano et al.
Expert opinion on investigational drugs, 23(7), 979-999 (2014-04-29)
IL-6 is a key cytokine in the pathogenesis of rheumatoid arthritis (RA). The clinical efficacy of tocilizumab (TCZ), a humanized anti-IL6-receptor mAb, confirmed the value of IL-6 blockade in this disease. A number of new anti-IL-6 biologics are currently in
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service