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P1253

Sigma-Aldrich

Phosvitin from egg yolk

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About This Item

CAS Number:
MDL number:
UNSPSC Code:
12352202
NACRES:
NA.81

Pricing and availability is not currently available.

biological source

egg yolk

Quality Level

form

powder

storage temp.

−20°C

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This Item
C0755A9275C7786
form

powder

form

powder

form

lyophilized powder

form

powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

General description

Phosvitin from egg yolk is a polyanionic phosphoserine rich protein.[1] This phosphoglycoprotein comprises α-helices and β-sheets structural elements, which are poorly organized due to the presence of phosphoserine.[1] It represents about 11% of yolk proteins and is hydrophilic.[2]

Application

Phosvitin from egg yolk has been used:
  • as a control protein for immobilization on-chip for deep purple protein staining methods for monitoring post-translation modifications[3]
  • as a substrate for casein kinase Iε in in vitro casein kinase assay[4]
  • as a standard in gel filtration chromatography (GFC) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) for quantification of phosvitin from egg extracts[5]
  • for immobilization onto Layer-by-Layer (LbL) for Fourier transform infrared spectroscopy-attenuated total reflectance (FTIR-ATR) studies[6]

Biochem/physiol Actions

Phosvitin fed to rats by adding egg yolk to their food resulted in decreased absorption of magnesium, iron and calcium but not of phosphorus. [7]
Phosvitin from egg yolk is a calcium chelator[1] and binds to iron present in the yolk.[2] The metal-binding functionality is exploited in mineral-binding bioactive peptide production.[2] It is a nutraceutical and has potential for phosphopeptides production.[2]

Other Notes

A phosophoprotein containing 8-10% phosphorus. Molar N/P ratio approx. 2.7.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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    K Abdelkebir et al.
    Acta biomaterialia, 8(9), 3419-3428 (2012-06-12)
    Carboxylated, sulfated and/or phosphorylated surfaces are admitted as potential optimal templates for biomimetic deposition of calcium phosphate (CaP) coatings in view of improving implants' osseointegration. Layer-by-layer films were built up consisting of anionic chondroitin sulfate (ChS), a biological carboxylated and
    Xiaowei Zhang et al.
    Journal of separation science, 34(22), 3295-3301 (2011-10-15)
    An attempt was made to develop a new protocol for preparing phosvitin that could be easy scaled up using polyethylene glycol (PEG6000). Influence of PEG6000 concentration and pH of sample solution on phosvitin isolation was investigated. Phosvitin of high purity
    Jun Sun et al.
    Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 915-916, 33-38 (2013-01-15)
    Functional immobilized metal affinity magnetic carboxymethyl chitosan nanoparticles (abbreviated as Fe(3)O(4) (PEG+CM-CTS) @ Fe (III)) were conveniently applied for phosvitin phosphopeptides (PPPs) enrichment for the first time. The morphology of magnetic nanoparticles was observed by transmission electron microscope (TEM). It
    Khalil Abdelkebir et al.
    Langmuir : the ACS journal of surfaces and colloids, 27(23), 14370-14379 (2011-10-20)
    Layer-by-Layer (LbL) coatings are promising tools for the biofunctionalization of biomaterials, as they allow stress-free immobilization of proteins. Here, we explore the possibility to immobilize phosvitin, a highly phosphorylated protein viewed as a model of bone phosphoproteins and, as such
    Shaohui Wang et al.
    The Journal of biological chemistry, 286(25), 22653-22664 (2011-05-03)
    How fish embryos that develop externally survive microbial attacks is poorly understood. Here, we clearly demonstrated that the embryo extract of zebrafish and its early embryo both displayed antimicrobial activity against microbes, including pathogenic Aeromonas hydrophila, and phosvitin (Pv), a

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