MX750S20

Mix-n-Stain^™ CF^™ 750 Antibody Labeling Kit (5-20μg)

• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• form: liquid
• Quality Level: 100
• storage condition: protect from light
• fluorescence: λ_ex 755 nm; λ_em 777 nm
• storage temp.: −20°C

Description

General description

This labeling kit is for labeling 5-20 μg antibody with the near infrared fluorescent dye CF750. Mix-n-Stain CF dye antibody labeling kits are a revolutionary antibody labeling technology that permits one to label antibodies with our superior CF dyes in 30 minutes without a purification step. The labeling procedure tolerates many common buffer components and antibody stabilizers. CF750 is one of our three near-IR CF dyes. It is superior to other similar near-IR dyes, such as Alexa Fluor^™ 750, IRDye^® and the DyLight^® near-IR dyes. All of our near- IR CF dyes have combined advantages in brightness, photostability, specificity and novel features ideal for in vivo imaging, near-IR Western blotting and flow cytometry.

Application

The Mix-n-Stain CF dye antibody labeling kit can be used to label primary antibody for ELISA, western blot, immunohistochemistry, flow cytometry, immunofluorescence or immunocytochemistry applications.

Features and Benefits

Mix-n-Stain CF dye antibody labeling kits dramatically simplify the process of preparing fluorescently labeled antibodies, particularly primary antibodies. Simply mix your antibody with a CF dye of your choice in the buffer provided, a step that takes less than 30 seconds of hands-on time. After waiting for another 30 minutes, without a separation step, you will have a fluorescent antibody conjugate that is as good as the best pre-labeled fluorescent antibody you can buy from some of the leading suppliers. There is no need to calculate how much dye you should use; just mix your antibody with the entire amount of dye provided and you will always produce optimal labeling. Moreover, the labeling reaction can tolerate the presence of common stabilizers, such as sodium azide, Tris, glycerol and low level of BSA.

Components

The kit consists of 1 vial each of Mix-n-Stain Dye, Mix-n-Stain Reaction Buffer (10x), Mix-n-Stain Storage Buffer, and an ultrafiltration vial.

Analysis Note

Direct replacement for: Alexa Fluor^™ 750, Cy^®7, DyLight^® 750, APC- Alexa Fluor^™ 750 , IRDye^® 750

Legal Information

This product is distributed by Sigma-Aldrich Co. under the authorization of Biotium, Inc. This product is covered by one or more US patents and corresponding patent claims outside the US patents or pending applications owned or licensed by Biotium, Inc. including without limitation: 12/334,387; 12/607,915; 12/699,778; 12/850,578; 61/454,484. In consideration of the purchase price paid by the buyer, the buyer is hereby granted a limited, non-exclusive, non-transferable license to use only the purchased amount of the product solely for the buyer′s own internal research in a manner consistent with the accompanying product literature. Except as expressly granted herein, the sale of this product does not grant to or convey upon the buyer any license, expressly, by implication or estoppel, under any patent right or other intellectual property right of Biotium, Inc. Buyer shall not resell or transfer this product to any third party, or use the product for any commercial purposes, including without limitation, any diagnostic, therapeutic or prophylactic uses. This product is for research use only. Any other uses, including diagnostic uses, require a separate license from Biotium, Inc. For information on purchasing a license to use this product for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.

ALEXA FLUOR is a trademark of Life Technologies

CF is a trademark of Biotium, Inc.

Cy is a registered trademark of Cytiva

DyLight is a registered trademark of Pierce Biotechnology, Inc.

IRDye is a registered trademark of LI-COR, Inc.

Mix-n-Stain is a trademark of Biotium, Inc.

Safety Information

• Pictograms: GHS05
• Signal Word: Warning
• Hazard Statements: H290 - H412
• Precautionary Statements: P234 - P273 - P390 - P501
• Hazard Classifications: Aquatic Chronic 3 - Met. Corr. 1
• Storage Class Code: 8A - Combustible, corrosive hazardous materials
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable