MBD0022

Inactivated Burkholderia pyrrocinia

Suitable for DNA extraction, PCR, sequencing and NGS, >10^8 bacteria/vial

• Synonym(s): PCR, Polymerase chain reaction

Properties

• Quality Level: 100
• form: powder
• concentration: >10^8 bacteria/vial
• technique(s): DNA extraction: suitable; DNA sequencing: suitable; PCR: suitable
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

Standardization of sample analysis is currently needed in microbiome genomics research workflow. Lack of standardization can lead to biases and errors in common processes during sample preparation and analysis such as sample amplification, sequencing and bioinformatics analyses.1 Inactivated Burkholderia pyrrocin can serve as standard for benchmarking the performance along the workflow of microbiomics or meta-genomics analyses and as a tool to increase reproducibility and allow comparison of results obtained by different labs.Burkholderia cepacia complex (Bcc) species are a group of Gram-negative opportunistic pathogens that chronically infect the airways of cystic fibrosis patients, but they can also infect patients with various types of immunosuppressive disorders. Bcc members are multidrug resistant bacteria that have the ability to persist in the infected host and also elicit robust inflammatory responses.2 It currently includes 20 closely related, but distinct, species of bacteria, with a wide environmental distribution.B. cepacia has an unusually large genetic make up with a total DNA content greater than 7 megabases and as many as three megabase-sized replicons. This large genetic make up accounts for its microbiological versatility.3 Advances in the taxonomy of Bcc revealed that it comprises at least nine genetically closely related species, including B. pyrrocinia, which can be differentiated on the basis of molecular and biochemical tests.4

Read here how to use our standards to ensure data integrity for your microbiome research.

Application

Inactivated bacteria are provided at >10^8 bacteria/vial as lyophilized powder.It is recommended to avoid freeze thaw cycles of this product.

Physical form

Powder -The inactivated bacteria are provided at >10^8 bacteria/vial as lyophilized powder

Reconstitution

It is recommended to dissolve the lyophilized inactivated bacteria in water (Sigma Cat#W4502) supplemented with at least 10% glycerol (Sigma Cat#G5516). Once dissolved it is recommended to aliquot immediately and store at -20°C for up to 1 month and avoid freeze thaw cycles

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3